TY - JOUR
T1 - Characterization of listeria monocytogenes isolates of food and human origins from Brazil using molecular typing procedures and in vitro cell culture assays
AU - Bueno, Valter F.
AU - Banerjee, Pratik
AU - Banada, Padmapriya P.
AU - José De Mesquita, Albenones
AU - Lemes-Marques, Eneida G.
AU - Bhunia, Arun K.
N1 - Funding Information:
Our thanks to CNPq and Capes agencies in Brazil for providing financial support to VFFB. We would also like to extend our sincere thanks to Dr John Patterson and Dr Mark Einstein of the Animal Sciences Department at Purdue University for help with phylogenetic analysis, Hyochin Kim for assistance with serotyping and Dr Eran Raizman and Kristin Burkholder for critical reading of the manuscript.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/2
Y1 - 2010/2
N2 - The spreading of diseases through foods is a worldwide concern. Here, molecular and in vitro cell-culture assays were employed to characterize 63 Brazilian Listeria monocytogenes isolates (food, 47; clinical, 16). Serotype 4b was the most predominant (49%) followed by 1/2b (30%), 1/2a (10%), 1/2c (6%), 3c (3%) and 3b (2%). Ribotyping yielded 17 ribopatterns, which were grouped into four phylogenetic clusters. Cluster A comprised of 39/63 isolates primarily of food origin, and clusters B, C and D contained both food and clinical isolates. Isolates were positive for virulence determinants prfA, hlyA and inlA: clinical isolates were more invasive to Caco-2 cells and expressed high levels of inlA transcripts than the food isolates. Highly invasive isolates also provoked more Ped-2E9 cells to die by apoptosis than the weakly-invasive strains. These data demonstrate a strong genetic relatedness among clinical and food isolates and suggest transmission of a subset of L. monocytogenes strains from food to humans.
AB - The spreading of diseases through foods is a worldwide concern. Here, molecular and in vitro cell-culture assays were employed to characterize 63 Brazilian Listeria monocytogenes isolates (food, 47; clinical, 16). Serotype 4b was the most predominant (49%) followed by 1/2b (30%), 1/2a (10%), 1/2c (6%), 3c (3%) and 3b (2%). Ribotyping yielded 17 ribopatterns, which were grouped into four phylogenetic clusters. Cluster A comprised of 39/63 isolates primarily of food origin, and clusters B, C and D contained both food and clinical isolates. Isolates were positive for virulence determinants prfA, hlyA and inlA: clinical isolates were more invasive to Caco-2 cells and expressed high levels of inlA transcripts than the food isolates. Highly invasive isolates also provoked more Ped-2E9 cells to die by apoptosis than the weakly-invasive strains. These data demonstrate a strong genetic relatedness among clinical and food isolates and suggest transmission of a subset of L. monocytogenes strains from food to humans.
KW - Cell-invasion
KW - Cytotoxicity
KW - Listeria monocytogenes
KW - Pathogenicity
KW - Polymerase-chain reaction
KW - Ribotyping
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U2 - 10.1080/09603120903281283
DO - 10.1080/09603120903281283
M3 - Article
C2 - 20104385
AN - SCOPUS:77149139472
SN - 0960-3123
VL - 20
SP - 43
EP - 59
JO - International Journal of Environmental Health Research
JF - International Journal of Environmental Health Research
IS - 1
ER -