Characterization of listeria monocytogenes isolates of food and human origins from Brazil using molecular typing procedures and in vitro cell culture assays

Valter F. Bueno, Pratik Banerjee, Padmapriya P. Banada, Albenones José De Mesquita, Eneida G. Lemes-Marques, Arun K. Bhunia

Research output: Contribution to journalArticlepeer-review

Abstract

The spreading of diseases through foods is a worldwide concern. Here, molecular and in vitro cell-culture assays were employed to characterize 63 Brazilian Listeria monocytogenes isolates (food, 47; clinical, 16). Serotype 4b was the most predominant (49%) followed by 1/2b (30%), 1/2a (10%), 1/2c (6%), 3c (3%) and 3b (2%). Ribotyping yielded 17 ribopatterns, which were grouped into four phylogenetic clusters. Cluster A comprised of 39/63 isolates primarily of food origin, and clusters B, C and D contained both food and clinical isolates. Isolates were positive for virulence determinants prfA, hlyA and inlA: clinical isolates were more invasive to Caco-2 cells and expressed high levels of inlA transcripts than the food isolates. Highly invasive isolates also provoked more Ped-2E9 cells to die by apoptosis than the weakly-invasive strains. These data demonstrate a strong genetic relatedness among clinical and food isolates and suggest transmission of a subset of L. monocytogenes strains from food to humans.

Original languageEnglish (US)
Pages (from-to)43-59
Number of pages17
JournalInternational Journal of Environmental Health Research
Volume20
Issue number1
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Keywords

  • Cell-invasion
  • Cytotoxicity
  • Listeria monocytogenes
  • Pathogenicity
  • Polymerase-chain reaction
  • Ribotyping

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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