Characterization of glutamyl-tRNA–dependent dehydratases using nonreactive substrate mimics

Ian R. Bothwell, Dillon P. Cogan, Terry Kim, Christopher J. Reinhardt, Wilfred Adrianus van der Donk, Satish K Nair

Research output: Contribution to journalArticle

Abstract

The peptide natural product nisin has been used as a food preservative for 6 decades with minimal development of resistance. Nisin contains the unusual amino acids dehydroalanine and dehydrobutyrine, which are posttranslationally installed by class I lanthipeptide dehydratases (LanBs) on a linear peptide substrate through an unusual glutamyl-tRNA–dependent dehydration of Ser and Thr. To date, little is known about how LanBs catalyze the transfer of glutamate from charged tRNAGlu to the peptide substrate, or how they carry out the subsequent elimination of the peptide-glutamyl adducts to afford dehydro amino acids. Here, we describe the synthesis of inert analogs that mimic substrate glutamyl-tRNAGlu and the glutamylated peptide intermediate, and determine the crystal structures of 2 LanBs in complex with each of these compounds. Mutational studies were used to characterize the function of the glutamylation and glutamate elimination active-site residues identified through the structural analysis. These combined studies provide insights into the mechanisms of substrate recognition, glutamylation, and glutamate elimination by LanBs to effect a net dehydration reaction of Ser and Thr.

Original languageEnglish (US)
Pages (from-to)17245-17250
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number35
DOIs
StatePublished - Aug 27 2019

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Hydro-Lyases
RNA, Transfer, Glu
Peptides
Nisin
Glutamic Acid
Dehydration
Food Preservatives
Amino Acids
Biological Products
Catalytic Domain

Keywords

  • Dehydratase
  • Lantibiotic
  • Mechanism
  • Nisin
  • X-ray crystallography

ASJC Scopus subject areas

  • General

Cite this

Characterization of glutamyl-tRNA–dependent dehydratases using nonreactive substrate mimics. / Bothwell, Ian R.; Cogan, Dillon P.; Kim, Terry; Reinhardt, Christopher J.; van der Donk, Wilfred Adrianus; Nair, Satish K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 35, 27.08.2019, p. 17245-17250.

Research output: Contribution to journalArticle

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