TY - JOUR
T1 - Characterization of genes involved in (p)ppGpp precursor biosynthesis in Erwinia amylovora
AU - Yang, Ho Wen
AU - Yu, Menghao
AU - Liu, Jun
AU - Khan, Awais
AU - Zhao, Youfu
N1 - Funding Information:
This project was supported by the Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2016-67013-24812 from the USDA National Institute of Food and Agriculture (YFZ).
Publisher Copyright:
© 2020, Società Italiana di Patologia Vegetale (S.I.Pa.V.).
PY - 2021/8
Y1 - 2021/8
N2 - Previous studies have shown that the hypersensitive response and pathogenicity (hrp)-type III secretion system (T3SS) is activated by the bacterial alarmone (p)ppGpp, which mediates the stringent response in nutrient starvation conditions in Erwinia amylovora. ATP and GTP, the precursors of (p)ppGpp biosynthesis, are synthesized from AMP/adenine and GMP/guanine, respectively, by the de novo pathway of purine biosynthesis. In this study, we examined the role of the purA/purB and guaA/guaB genes, which are involved in the AMP/adenine and GMP/guanine biosynthesis, respectively. Our results showed that growth of the purA, purB, and guaAB mutants was much slower in minimal media, but could be restored by addition of exogenous AMP/adenine and GMP/guanine, respectively. However, mixed results were obtained for their contribution to virulence on immature pear fruits. These results suggested that the de novo purine biosynthesis pathway might be required for proliferation, colonization and infection of E. amylovora.
AB - Previous studies have shown that the hypersensitive response and pathogenicity (hrp)-type III secretion system (T3SS) is activated by the bacterial alarmone (p)ppGpp, which mediates the stringent response in nutrient starvation conditions in Erwinia amylovora. ATP and GTP, the precursors of (p)ppGpp biosynthesis, are synthesized from AMP/adenine and GMP/guanine, respectively, by the de novo pathway of purine biosynthesis. In this study, we examined the role of the purA/purB and guaA/guaB genes, which are involved in the AMP/adenine and GMP/guanine biosynthesis, respectively. Our results showed that growth of the purA, purB, and guaAB mutants was much slower in minimal media, but could be restored by addition of exogenous AMP/adenine and GMP/guanine, respectively. However, mixed results were obtained for their contribution to virulence on immature pear fruits. These results suggested that the de novo purine biosynthesis pathway might be required for proliferation, colonization and infection of E. amylovora.
KW - (p)ppGpp
KW - Gene regulation
KW - Type III secretion
KW - Virulence
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U2 - 10.1007/s42161-020-00583-6
DO - 10.1007/s42161-020-00583-6
M3 - Article
AN - SCOPUS:85086394621
VL - 103
SP - 79
EP - 88
JO - Journal of Plant Pathology
JF - Journal of Plant Pathology
SN - 1125-4653
ER -