Characterization of estrogen receptor β2 and expression of the estrogen receptor subtypes α, β1, and β2 in the protandrous black porgy (Acanthopagrus schlegeli) during the sex change process

Kwang Wook An, Erik R. Nelson, Pil Gue Jo, Hamid R. Habibi, Hyun Suk Shin, Cheol Young Choi

Research output: Contribution to journalArticle

Abstract

Estrogens play an important role in many physiological processes in both female and male vertebrates, mediated by specific nuclear receptor, estrogen receptors (ERs). We have isolated a third ER (ERβ2), which was found to contain 2004 nucleotides including an open reading frame that encodes 667 amino acids. We have also cloned ERα and ERβ1 from the published information (GenBank accession nos. AY074780 and AY074779) and investigated the expression pattern of these ER subtypes in the gonads during gonad sex change of black porgy by quantitative polymerase chain reaction. Maturity stages can be divided into five stages during the sex change process from immature male to female (immature male, mature male, male of mostly testis, male of mostly ovary and mature female). The expression of ERα mRNA was highest in the ovary of mature female, followed by the testis of mature male and testicular portion of mostly testis. ERβ1 expression was higher in the mature testis and ovary than in the gonads of other maturity stages. In contrast to that, ERβ2 was highest in the ovary of mature female, and significantly lower levels of ERβ2 expression were observed in the gonads of the other maturity stages. The present study describes the molecular characterization of ERβ2, and documents the expression changes of three ER subtypes during sex change process of the protandrous black porgy.

Original languageEnglish (US)
Pages (from-to)284-291
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume150
Issue number3
DOIs
StatePublished - Jul 1 2008

Fingerprint

Estrogen Receptor beta
Estrogen Receptor alpha
Estrogen Receptors
Gonads
Testis
Ovary
Physiological Phenomena
Polymerase chain reaction
Nucleic Acid Databases
Cytoplasmic and Nuclear Receptors
Estrogens
Open Reading Frames
Nucleotides
Vertebrates
Amino Acids
Messenger RNA
Polymerase Chain Reaction

Keywords

  • Black porgy
  • Estrogen receptor
  • Histology
  • Protandrous fish
  • Sex change

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

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title = "Characterization of estrogen receptor β2 and expression of the estrogen receptor subtypes α, β1, and β2 in the protandrous black porgy (Acanthopagrus schlegeli) during the sex change process",
abstract = "Estrogens play an important role in many physiological processes in both female and male vertebrates, mediated by specific nuclear receptor, estrogen receptors (ERs). We have isolated a third ER (ERβ2), which was found to contain 2004 nucleotides including an open reading frame that encodes 667 amino acids. We have also cloned ERα and ERβ1 from the published information (GenBank accession nos. AY074780 and AY074779) and investigated the expression pattern of these ER subtypes in the gonads during gonad sex change of black porgy by quantitative polymerase chain reaction. Maturity stages can be divided into five stages during the sex change process from immature male to female (immature male, mature male, male of mostly testis, male of mostly ovary and mature female). The expression of ERα mRNA was highest in the ovary of mature female, followed by the testis of mature male and testicular portion of mostly testis. ERβ1 expression was higher in the mature testis and ovary than in the gonads of other maturity stages. In contrast to that, ERβ2 was highest in the ovary of mature female, and significantly lower levels of ERβ2 expression were observed in the gonads of the other maturity stages. The present study describes the molecular characterization of ERβ2, and documents the expression changes of three ER subtypes during sex change process of the protandrous black porgy.",
keywords = "Black porgy, Estrogen receptor, Histology, Protandrous fish, Sex change",
author = "An, {Kwang Wook} and Nelson, {Erik R.} and Jo, {Pil Gue} and Habibi, {Hamid R.} and Shin, {Hyun Suk} and Choi, {Cheol Young}",
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T1 - Characterization of estrogen receptor β2 and expression of the estrogen receptor subtypes α, β1, and β2 in the protandrous black porgy (Acanthopagrus schlegeli) during the sex change process

AU - An, Kwang Wook

AU - Nelson, Erik R.

AU - Jo, Pil Gue

AU - Habibi, Hamid R.

AU - Shin, Hyun Suk

AU - Choi, Cheol Young

PY - 2008/7/1

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N2 - Estrogens play an important role in many physiological processes in both female and male vertebrates, mediated by specific nuclear receptor, estrogen receptors (ERs). We have isolated a third ER (ERβ2), which was found to contain 2004 nucleotides including an open reading frame that encodes 667 amino acids. We have also cloned ERα and ERβ1 from the published information (GenBank accession nos. AY074780 and AY074779) and investigated the expression pattern of these ER subtypes in the gonads during gonad sex change of black porgy by quantitative polymerase chain reaction. Maturity stages can be divided into five stages during the sex change process from immature male to female (immature male, mature male, male of mostly testis, male of mostly ovary and mature female). The expression of ERα mRNA was highest in the ovary of mature female, followed by the testis of mature male and testicular portion of mostly testis. ERβ1 expression was higher in the mature testis and ovary than in the gonads of other maturity stages. In contrast to that, ERβ2 was highest in the ovary of mature female, and significantly lower levels of ERβ2 expression were observed in the gonads of the other maturity stages. The present study describes the molecular characterization of ERβ2, and documents the expression changes of three ER subtypes during sex change process of the protandrous black porgy.

AB - Estrogens play an important role in many physiological processes in both female and male vertebrates, mediated by specific nuclear receptor, estrogen receptors (ERs). We have isolated a third ER (ERβ2), which was found to contain 2004 nucleotides including an open reading frame that encodes 667 amino acids. We have also cloned ERα and ERβ1 from the published information (GenBank accession nos. AY074780 and AY074779) and investigated the expression pattern of these ER subtypes in the gonads during gonad sex change of black porgy by quantitative polymerase chain reaction. Maturity stages can be divided into five stages during the sex change process from immature male to female (immature male, mature male, male of mostly testis, male of mostly ovary and mature female). The expression of ERα mRNA was highest in the ovary of mature female, followed by the testis of mature male and testicular portion of mostly testis. ERβ1 expression was higher in the mature testis and ovary than in the gonads of other maturity stages. In contrast to that, ERβ2 was highest in the ovary of mature female, and significantly lower levels of ERβ2 expression were observed in the gonads of the other maturity stages. The present study describes the molecular characterization of ERβ2, and documents the expression changes of three ER subtypes during sex change process of the protandrous black porgy.

KW - Black porgy

KW - Estrogen receptor

KW - Histology

KW - Protandrous fish

KW - Sex change

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