Characterization of bimodal coordination structure in nitrosyl heme complexes through hyperfine couplings with pyrrole and protein nitrogens

A. M. Tyryshkin, S. A. Dikanov, E. J. Reijerse, C. Burgard, J. Hüttermann

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Orientation-selected three-pulse ESEEM experiments have been performed on a series of nitrosyl hemoproteins: HbNO in its two quaternary (R/T) states, the isolated NO-ligated α(β)-chains of hemoglobin, two hybrids of hemoglobin with asymmetrically ligated α(β)-chains, NO-myoglobin, and NO- Fe2+(TPP)-imidazole model complexes. The ESEEM spectra of the native complexes clearly revealed the contribution from two conformational states of the NO-heme group. At 4.2 K the αNO and βNO chains were found in an almost pure state, i.e., 80% 'state I' and 90% 'state II', respectively. These results correlate well with the two-conformation model of 6-coordinated NO- heme complexes proposed earlier from the evaluation of temperature-dependent EPR/ENDOR spectra (Morse, R. H.; Chan, S. I. J. Biol. Chem. 1980, 255, 7876. Huttermann, J.; Burgard, C.; Kappl, R. J. Chem. Soc., Faraday Trans. 1994, 90, 3077). Application of two-dimensional ESEEM spectroscopy (HYSCORE) to the isolated αNO and βNO chains allowed the characterization of the pyrrole nitrogen HFI in both conformations. A third nitrogen coupling was identified in the HYSCORE of the βNO chain. It was tentatively assigned to the N(ε) nitrogen of distal His E7 which is suggested to form a hydrogen bond to the NO group in the axial NO-heme conformation. These findings support the proposal that the variation of binding geometry in two states of NO-heme is controlled by the heme's protein surrounding and could provide an important contribution to the discussion on the physiological role of NO related to its interactions with protein metal centers.

Original languageEnglish (US)
Pages (from-to)3396-3406
Number of pages11
JournalJournal of the American Chemical Society
Issue number14
StatePublished - Apr 14 1999

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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