Abstract

Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. From B. subtilis cells cultivated on a biofilm-promoting medium, we detected two cannibalistic factors not found in previous MALDI MSI studies of the same strain under different culturing conditions. Given the importance of cannibalism in matrix formation of B. subtilis biofilms, we employed a transcriptional reporter to monitor matrix-producing cell subpopulations using fluorescence imaging. These two complementary imaging approaches were used to characterize three B. subtilis strains, the wild type isolate NCIB3610, and two mutants, Δspo0A and ΔabrB, with defective and enhanced biofilm phenotypes, respectively. Upon deletion of key transcriptional factors, correlated changes were observed in biofilm morphology, signaling, cannibalistic factor distribution, and matrix-related gene expression, providing new insights on cannibalism in biofilm development. This work underscores the advantages of using multimodal imaging to compare spatial patterns of selected molecules with the associated protein expression patterns, obtaining information on cellular heterogeneity and function not obtainable when using a single method to characterize biofilm formation.

Original languageEnglish (US)
Pages (from-to)1955-1962
Number of pages8
JournalJournal of Proteome Research
Volume15
Issue number6
DOIs
StatePublished - Jun 3 2016

Fingerprint

Optical Imaging
Biofilms
Bacilli
Bacillus subtilis
Mass spectrometry
Mass Spectrometry
Fluorescence
Imaging techniques
Cannibalism
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ionization
Desorption
Multimodal Imaging
Lasers
Gene expression
Phenotype
Gene Expression
Molecules

Keywords

  • biofilm
  • cell differentiation
  • fluorescence imaging
  • mass spectrometry imaging
  • transcriptional reporter

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging. / Si, Tong; Li, Bin; Zhang, Ke; Xu, Yiran; Zhao, Huimin; Sweedler, Jonathan V.

In: Journal of Proteome Research, Vol. 15, No. 6, 03.06.2016, p. 1955-1962.

Research output: Contribution to journalArticle

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