Characterization of aqueous phase from the hydrothermal liquefaction of Chlorella pyrenoidosa

Chao Gai, Yuanhui Zhang, Wan Ting Chen, Yan Zhou, Lance Schideman, Peng Zhang, Giovana Tommaso, Chih Ting Kuo, Yuping Dong

Research output: Contribution to journalArticlepeer-review


This study investigated the characteristics of aqueous phase from hydrothermal liquefaction of low-lipid microalgae Chlorella pyrenoidosa. The interactions of operating conditions, including reaction temperature, retention time and total solid ratio were evaluated by response surface methodology. The chemical oxygen demand, total nitrogen and total phosphorus were selected as indicators of the property of AP. Results indicated that total solid ratio was found to be the dominant factor affecting the nutrient recovery efficiencies of AP. Based on energy recovery, GC-MS indicated that the AP at two optimized operating conditions (280. °C, 60. min, 35. wt.% and 300. °C, 60. min, 25. wt.%) were observed to have a higher concentration of organic acids (10.35% and 8.34%) while the sample (260. °C, 30. min, 35. wt.%) was observed to have the highest concentration of N&O-heterocyclic compounds (36.16%).

Original languageEnglish (US)
Pages (from-to)328-335
Number of pages8
JournalBioresource Technology
StatePublished - May 1 2015


  • Aqueous product
  • Hydrothermal liquefaction
  • Low-lipid algae
  • Nitrogen recovery
  • Nutrient

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal


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