Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets

Sayed Zahirul Islam; Yuichi Honda; Yoshihiro Sawa; Mohammad Babadoost

doi:10.1007/s102670200068

Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets

Sayed Zahirul Islam, Yuichi Honda, Yoshihiro Sawa, Mohammad Babadoost

Research output: Contribution to journal › Article › peer-review

Abstract

Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO₄), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.

Original language	English (US)
Pages (from-to)	471-473
Number of pages	3
Journal	Mycoscience
Volume	43
Issue number	6
DOIs	https://doi.org/10.1007/s102670200068
State	Published - Dec 25 2002

Keywords

Antifungal substance
Glycoprotein
Red light
Vicia faba

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

Online availability

10.1007/s102670200068

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abstract = "Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.",

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AB - Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.

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Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets

Abstract

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