Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets

Sayed Zahirul Islam, Yuichi Honda, Yoshihiro Sawa, Mohammad Babadoost

Research output: Contribution to journalArticlepeer-review


Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.

Original languageEnglish (US)
Pages (from-to)471-473
Number of pages3
Issue number6
StatePublished - Dec 25 2002


  • Antifungal substance
  • Glycoprotein
  • Red light
  • Vicia faba

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics


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