Abstract
Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.
Original language | English (US) |
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Pages (from-to) | 471-473 |
Number of pages | 3 |
Journal | Mycoscience |
Volume | 43 |
Issue number | 6 |
DOIs | |
State | Published - Dec 25 2002 |
Keywords
- Antifungal substance
- Glycoprotein
- Red light
- Vicia faba
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics