TY - JOUR
T1 - Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets
AU - Zahirul Islam, Sayed
AU - Honda, Yuichi
AU - Sawa, Yoshihiro
AU - Babadoost, Mohammad
PY - 2002/12/25
Y1 - 2002/12/25
N2 - Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.
AB - Red-light treatment of broadbean leaflets resulted in the production of antifungal substance(s) against Botrytis cinerea. The antifungal substance(s) was positively charged, as the antifungal constituent was removed by the cation exchanger CM cellulose. Treatment of infection droplets with glycosidases (α-mannosidase, β-galactosidase, β-glucosidase), glycol-specific reagent periodate (NaIO4), and proteinase K completely eliminated antifungal activity, suggesting that both protein and carbohydrate are active components. The protein content of infection droplets was 0.148mg/ml. The HPLC gel column analysis of infection droplets resulted in four fractions; all the fractions showed antifungal activity.
KW - Antifungal substance
KW - Glycoprotein
KW - Red light
KW - Vicia faba
UR - http://www.scopus.com/inward/record.url?scp=0037176421&partnerID=8YFLogxK
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U2 - 10.1007/s102670200068
DO - 10.1007/s102670200068
M3 - Article
AN - SCOPUS:0037176421
SN - 1340-3540
VL - 43
SP - 471
EP - 473
JO - Mycoscience
JF - Mycoscience
IS - 6
ER -