Characterization of anthocyanins and proanthocyanidins in wild and domesticated mexican blackberries (Rubus spp.)

Edith O. Cuevas-Rodríguez, Gad G. Yousef, Pedro A. García-Saucedo, José López-Medina, Octavio Paredes-López, Mary Ann Lila

Research output: Contribution to journalArticlepeer-review

Abstract

This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacán, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated breeding line), were selected and profiled for anthocyanins and proanthocyanidins by separating extracts over Amberlite XAD-7 resin and Sephadex LH-20 columns. Subsequent high-performance liquid chromatography (HPLC) and liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that the major anthocyanin for all genotypes was cyanidin 3-O-glucoside. The proanthocyanidins (condensed tannins) were present in mono-to hexamer forms. Also, hydrolyzable tannins, ellagitannins, were characterized in the blackberry fruits. The average anthocyanin concentration in Sephadex LH-20 fractions was 49.2 mg/g in the commercial cultivar Tupy, while in the wild genotypes and the breeding line, the range was 361.3-494.9 mg/g (cyanidin 3-O-glucoside equivalent). The proanthocyanidin concentration varied widely among wild genotypes (417.5-1343.6 mg/g, catechin equivalent). This study demonstrated that the use of Amberlite XAD-7 followed by Sephadex LH-20 chromatography, with subsequent HPLC and LC-ESI-MS analyses, was able to effectively separate and characterize the diverse polyphenolics in blackberry genotypes. These results suggest that recommendations for dietary intake of blackberries for human health benefits need to take into account the source, because of the wide inherent variation in bioactive polyphenolic content in different blackberry genotypes.

Original languageEnglish (US)
Pages (from-to)7458-7464
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number12
DOIs
StatePublished - Jun 23 2010

Keywords

  • Anthocyanin
  • Ellagitannins
  • HPLC-ESI-MS
  • Proanthocyanidin
  • Rubus

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Characterization of anthocyanins and proanthocyanidins in wild and domesticated mexican blackberries (Rubus spp.)'. Together they form a unique fingerprint.

Cite this