TY - JOUR
T1 - Characterization of an emerging porcine respirovirus 1 isolate in the US
T2 - A novel viral vector for expression of foreign antigens
AU - Li, Yanhua
AU - Yuan, Fangfeng
AU - Yan, Xingyu
AU - Matta, Tori
AU - Cino-Ozuna, Giselle A.
AU - Fang, Ying
N1 - This project was supported by research startup funds (to Y. Fang) at Kansas State University and University of Illnois at Urbana-Champaign. Fangfeng Yuan was partially supported by Illinois Distinguished Fellowship for graduate student at University of Illinois .
PY - 2022/5
Y1 - 2022/5
N2 - Porcine respirovirus 1 (PRV1) is widely spread in many countries. In this study, we isolated an emgerging PRV1 strain (KS17-258) from a US swine farm. A full-length genome sequence of the virus was obtained, and the mRNA editing mechanism utilized for the expression of V/W proteins by P gene was confirmed. The virus shares 91.3–98% nucleotide sequence identity with the other PRV1 genomes reported previously. Phylogenetic analysis showed that KS17-258 forms a clade with the other US isolates. Infectious clone of the KS17-258 isolate was constructed, which was further explored as a viral vector to express enhanced green fluorescent protein (EGFP). The expression cassette of EGFP in the recombinant virus remained stable for 10 passages in cell culture. The availability of PRV1 infectious clone provides an important tool for study the basic PRV1 replication mechanisms. It also provides a novel platform for potential development of vectored vaccines against swine diseases.
AB - Porcine respirovirus 1 (PRV1) is widely spread in many countries. In this study, we isolated an emgerging PRV1 strain (KS17-258) from a US swine farm. A full-length genome sequence of the virus was obtained, and the mRNA editing mechanism utilized for the expression of V/W proteins by P gene was confirmed. The virus shares 91.3–98% nucleotide sequence identity with the other PRV1 genomes reported previously. Phylogenetic analysis showed that KS17-258 forms a clade with the other US isolates. Infectious clone of the KS17-258 isolate was constructed, which was further explored as a viral vector to express enhanced green fluorescent protein (EGFP). The expression cassette of EGFP in the recombinant virus remained stable for 10 passages in cell culture. The availability of PRV1 infectious clone provides an important tool for study the basic PRV1 replication mechanisms. It also provides a novel platform for potential development of vectored vaccines against swine diseases.
KW - PRV1 infectious clone
KW - PRV1 viral vector
KW - Porcine parainfluenza virus 1
KW - Porcine respirovirus 1
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U2 - 10.1016/j.virol.2022.03.013
DO - 10.1016/j.virol.2022.03.013
M3 - Article
C2 - 35398774
AN - SCOPUS:85127500696
SN - 0042-6822
VL - 570
SP - 107
EP - 116
JO - Virology
JF - Virology
ER -