@inproceedings{9b8f133edb714d57b0ee3afdd330d2e7,
title = "Characterization of a reflective objective with multiphoton microscopy",
abstract = "Reflective objectives (ROs) can reduce chromatic aberration across a wide wavelength range in multiphoton microscopy (MPM). However, a systematic characterization of the performance of ROs has not been carried out. In this paper, we analyze the performance of a 0.5 numerical-aperture (NA) RO and compare it with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments extending ∼1 octave in visible and NIR wavelengths, the SO introduces defocusing errors of ∼25\% for TPF images of sub-diffraction fluorescent beads and ∼10\% for SHG images of collagen fibers. For both imaging systems, the RO provides a corresponding error of ∼4\%. This work highlights the potential usefulness of ROs for multimodal MPM applications.",
keywords = "multiphoton microscopy, reflective objective, second-harmonic generation, two-photon fluorescence",
author = "Kabir, \{Mohammad M.\} and Choubal, \{Aakash M.\} and Mayandi Sivaguru and Toussaint, \{Kimani C.\}",
note = "This work was supported by Carver Trust (15-4588). We thank Woowon Lee for help with tendon sample preparation and Eric Seabron for help with optical microscopy setup.; Multiphoton Microscopy in the Biomedical Sciences XVIII 2018 ; Conference date: 28-01-2018 Through 30-01-2018",
year = "2018",
doi = "10.1117/12.2290305",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ammasi Periasamy and Xie, \{Xiaoliang S.\} and Xie, \{Xiaoliang S.\} and Karsten Konig and So, \{Peter T. C.\}",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XVIII",
}