Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death

Michelle E. Montemayor; Susan E. Fahrbach; Carol S. Giometti; Edward J. Roy

doi:10.1016/0014-5793(90)80546-U

Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death

Michelle E. Montemayor, Susan E. Fahrbach, Carol S. Giometti, Edward J. Roy

Research output: Contribution to journal › Article › peer-review

Abstract

Two-dimensional gel electrophoresis was used to locate potential neuronal death-related proteins in the moth Manduca sexta. Protein patterns of ganglia of pharate adult moths (taken prior to adult ecdysis) compared with protein patterns of one-day-old adults revealed reproducible changes in protein patterns. An acidic protein of approximately 40000 Da was present in all samples from adult moths undergoing neuronal death and essentially absent from pharate adult samples.

Original language	English (US)
Pages (from-to)	219-222
Number of pages	4
Journal	FEBS Letters
Volume	276
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)80546-U
State	Published - Dec 10 1990

Keywords

Abdominal ganglia
Cell death
Gel electrophoresis, 2-D
Manduca sexta
Metamorphosis
Neuronal death
Protein synthesis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/0014-5793(90)80546-U

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title = "Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death",

abstract = "Two-dimensional gel electrophoresis was used to locate potential neuronal death-related proteins in the moth Manduca sexta. Protein patterns of ganglia of pharate adult moths (taken prior to adult ecdysis) compared with protein patterns of one-day-old adults revealed reproducible changes in protein patterns. An acidic protein of approximately 40000 Da was present in all samples from adult moths undergoing neuronal death and essentially absent from pharate adult samples.",

keywords = "Abdominal ganglia, Cell death, Gel electrophoresis, 2-D, Manduca sexta, Metamorphosis, Neuronal death, Protein synthesis",

author = "Montemayor, {Michelle E.} and Fahrbach, {Susan E.} and Giometti, {Carol S.} and Roy, {Edward J.}",

note = "Funding Information: AcknowledgementsW: e thankS andraT ollaksena nd Anne Gemmell for technicala ssistanceT. his study was supportedi n part by NSF BNS 86-1677t7o E.J.R. and by theU S Departmenot f Energy,O ffice of Health and EnvironmentalR esearchu nder contract number W-31-109-ENG-38.M .E.M. is supported by NIMH Behavioral NeuroscienceP ostdoctoral Fellowship PHS 5 P32T32 MH18412. S.E.F. is an Alfred P. Sloan ResearchF ellow.",

year = "1990",

month = dec,

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doi = "10.1016/0014-5793(90)80546-U",

language = "English (US)",

volume = "276",

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journal = "FEBS Letters",

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number = "1-2",

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TY - JOUR

T1 - Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death

AU - Montemayor, Michelle E.

AU - Fahrbach, Susan E.

AU - Giometti, Carol S.

AU - Roy, Edward J.

N1 - Funding Information: AcknowledgementsW: e thankS andraT ollaksena nd Anne Gemmell for technicala ssistanceT. his study was supportedi n part by NSF BNS 86-1677t7o E.J.R. and by theU S Departmenot f Energy,O ffice of Health and EnvironmentalR esearchu nder contract number W-31-109-ENG-38.M .E.M. is supported by NIMH Behavioral NeuroscienceP ostdoctoral Fellowship PHS 5 P32T32 MH18412. S.E.F. is an Alfred P. Sloan ResearchF ellow.

PY - 1990/12/10

Y1 - 1990/12/10

N2 - Two-dimensional gel electrophoresis was used to locate potential neuronal death-related proteins in the moth Manduca sexta. Protein patterns of ganglia of pharate adult moths (taken prior to adult ecdysis) compared with protein patterns of one-day-old adults revealed reproducible changes in protein patterns. An acidic protein of approximately 40000 Da was present in all samples from adult moths undergoing neuronal death and essentially absent from pharate adult samples.

AB - Two-dimensional gel electrophoresis was used to locate potential neuronal death-related proteins in the moth Manduca sexta. Protein patterns of ganglia of pharate adult moths (taken prior to adult ecdysis) compared with protein patterns of one-day-old adults revealed reproducible changes in protein patterns. An acidic protein of approximately 40000 Da was present in all samples from adult moths undergoing neuronal death and essentially absent from pharate adult samples.

KW - Abdominal ganglia

KW - Cell death

KW - Gel electrophoresis, 2-D

KW - Manduca sexta

KW - Metamorphosis

KW - Neuronal death

KW - Protein synthesis

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ER -

Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death

Abstract

Keywords

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