Characterization of a new insect cell line (NTU-YB) derived from the common grass yellow butterfly, Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera) and its susceptibility to microsporidia

Yun Ru Chen, Leellen F. Solter, Tsz Ying Chien, Ming Han Jiang, Hsieh Fang Lin, Huai Sheng Fan, Chu Fang Lo, Chung Hsiung Wang

Research output: Contribution to journalArticlepeer-review

Abstract

A new lepidopteran cell line, NTU-YB, was derived from pupal tissue of Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera). The doubling time of YB cells in TNM-FH medium supplemented with 8% FBS at 28 °C was 26.87 h. The chromosome numbers of YB cells varied widely from 21 to 196 with a mean of 86. Compared to other insect cell lines, the YB cells produced distinct esterase, malate dehydrogenase, and lactate dehydrogenase isozyme patterns. Identity of the internal transcribed spacer region-I (ITS-I) of YB cells to E. hecabe larvae was 96% and to Eurema blanda larvae (tissue isolated from head) was 81%. The YB cells were permissive to Nosema sp. isolated from E. blanda and the infected YB cells showed obvious cytopathic effects after 3 weeks post inoculation. The highest level of spore production was at 4 weeks post inoculation when cells were infected with the Nosema isolate, and spore production was 1.34 ± 0.9 × 106 spore/ml. Ultrastructrual studies showed that YB cells can host in vitro propagation of the E. blanda Nosema isolate, and developing stages were observed in the host cell nuclei as observed in the natural host, E. blanda. The NTU-YB cell line is also susceptible to Nosema bombycis.

Original languageEnglish (US)
Pages (from-to)256-262
Number of pages7
JournalJournal of Invertebrate Pathology
Volume102
Issue number3
DOIs
StatePublished - Nov 1 2009

Keywords

  • Eurema blanda
  • Eurema hecabe
  • Microsporidia
  • NTU-YB cell line
  • Nosema sp.

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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