Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine

Diana A. Stavreva; Elizabeth D. Wagner; Michael J. Plewa; Tomáš Gichner

doi:10.1016/S0027-5107(97)00137-1

Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine

Diana A. Stavreva, Elizabeth D. Wagner, Michael J. Plewa, Tomáš Gichner

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The medium recovered from the tobacco cell suspension cultures (TX1MX) activated the promutagenic aromatic amine m-phenylenediamine (mPDA) and a macromolecular complex (gel) responsible for the arylamine activation was isolated from the medium. The gel formation and the role of the gel components in the plant activation of mPDA to products mutagenic in S. typhimurium YG1024 were studied. The activation of mPDA was caused by the peroxidases present in TX1MX. We demonstrated an association of the peroxidase activity and gel pectins. Formation of a stable mutagenic association of mPDA with the macromolecular material was observed. The data indicate that the gel isolated from TX1MX is the macromolecular component of the arylamine conjugate proposed in earlier work.

Original language	English (US)
Pages (from-to)	191-199
Number of pages	9
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	379
Issue number	2
DOIs	https://doi.org/10.1016/S0027-5107(97)00137-1
State	Published - Oct 6 1997

Keywords

S. typhimurium
YG1024

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/S0027-5107(97)00137-1

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Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine. / Stavreva, Diana A.; Wagner, Elizabeth D.; Plewa, Michael J. et al.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 379, No. 2, 06.10.1997, p. 191-199.

Research output: Contribution to journal › Article › peer-review

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title = "Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine",

abstract = "The medium recovered from the tobacco cell suspension cultures (TX1MX) activated the promutagenic aromatic amine m-phenylenediamine (mPDA) and a macromolecular complex (gel) responsible for the arylamine activation was isolated from the medium. The gel formation and the role of the gel components in the plant activation of mPDA to products mutagenic in S. typhimurium YG1024 were studied. The activation of mPDA was caused by the peroxidases present in TX1MX. We demonstrated an association of the peroxidase activity and gel pectins. Formation of a stable mutagenic association of mPDA with the macromolecular material was observed. The data indicate that the gel isolated from TX1MX is the macromolecular component of the arylamine conjugate proposed in earlier work.",

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author = "Stavreva, {Diana A.} and Wagner, {Elizabeth D.} and Plewa, {Michael J.} and Tom{\'a}{\v s} Gichner",

note = "This research was funded in part by US Environmental Protection Agency Research Grant No. R-823184 (MJP), US Czech Science and Technology Program Grant No. 930-13 (TG and MJP), the EU Copernicus Grant No. CIPA-CT94-0133 (TG) and by the Grant Agency of the Czech Republic, Grant No. 506-95-0084 (TG).",

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AU - Gichner, Tomáš

N1 - This research was funded in part by US Environmental Protection Agency Research Grant No. R-823184 (MJP), US Czech Science and Technology Program Grant No. 930-13 (TG and MJP), the EU Copernicus Grant No. CIPA-CT94-0133 (TG) and by the Grant Agency of the Czech Republic, Grant No. 506-95-0084 (TG).

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N2 - The medium recovered from the tobacco cell suspension cultures (TX1MX) activated the promutagenic aromatic amine m-phenylenediamine (mPDA) and a macromolecular complex (gel) responsible for the arylamine activation was isolated from the medium. The gel formation and the role of the gel components in the plant activation of mPDA to products mutagenic in S. typhimurium YG1024 were studied. The activation of mPDA was caused by the peroxidases present in TX1MX. We demonstrated an association of the peroxidase activity and gel pectins. Formation of a stable mutagenic association of mPDA with the macromolecular material was observed. The data indicate that the gel isolated from TX1MX is the macromolecular component of the arylamine conjugate proposed in earlier work.

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Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine

Abstract

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