Characterization of a Glyphosate-Tolerant Enzyme from Streptomyces svecius: A Distinct Class of 5-Enolpyruvylshikimate-3-phosphate Synthases

Samantha L. Griffin, Jonathan R. Chekan, Justin M. Lira, Andrew E. Robinson, Carla N. Yerkes, Daniel L. Siehl, Terry R. Wright, Satish K. Nair, Robert M. Cicchillo

Research output: Contribution to journalArticlepeer-review

Abstract

Natural and modified versions of the 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene have been used to confer tolerance to the broad-spectrum herbicide glyphosate in a variety of commercial crops. The most widely utilized trait was obtained from the Agrobacterium tumefaciens strain CP4 and has been commercialized in several glyphosate-tolerant crops. The EPSPS gene products are enzymes that have been divided into three classes based on sequence similarity, sensitivity to glyphosate, and steady-state catalytic parameters. Herein, we describe the informatics-guided identification and biochemical and structural characterization of a novel EPSPS from Streptomyces sviceus (DGT-28 EPSPS). The data suggest DGT-28 EPSPS and other closely related homologues exemplify a distinct new class (Class IV) of EPSPS enzymes that display intrinsic tolerance to high concentrations of glyphosate (Ki ≥ 5000 μM). We further demonstrate that dgt-28 epsps, when transformed into stable plants, provides robust (≥4× field rates) vegetative/reproductive herbicide tolerance and has utility in weed-control systems comparable to that of commercialized events.

Original languageEnglish (US)
Pages (from-to)5096-5104
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number17
DOIs
StatePublished - May 5 2021

Keywords

  • EPSPS
  • X-ray crystallography
  • enzyme kinetics
  • glyphosate
  • herbicide tolerance
  • shikimate pathway

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint

Dive into the research topics of 'Characterization of a Glyphosate-Tolerant Enzyme from Streptomyces svecius: A Distinct Class of 5-Enolpyruvylshikimate-3-phosphate Synthases'. Together they form a unique fingerprint.

Cite this