@article{912f9e257f2644f6967dbd21d76bf735,
title = "Characterization of 29 polymorphic microsatellite markers developed by genomic screening of Sumatran rhinoceros (Dicerorhinus sumatrensis)",
abstract = "Objective: The Sumatran rhinoceros is critically endangered, with fewer than 100 individuals surviving across its current range. Accurate census estimates of the remaining populations are essential for development and implementation of conservation plans. In order to enable molecular censusing, we here develop microsatellite markers with amplicon sizes of short length, appropriate for non-invasive fecal sampling. Results: Due to limited sample quantity and potential lack of genome-wide diversity, Illumina sequence reads were generated from two Sumatran rhinoceros samples. Genomic screening identified reads with short tandem repeats and loci that were polymorphic within the dataset. Twenty-nine novel polymorphic microsatellite markers were characterized (A = 2.4; HO = 0.30). These were sufficient to distinguish among individuals (PID < 0.0001), and to distinguish among siblings (PID(sib) < 0.0001). Among rhinos in Indonesia, almost all markers were established as polymorphic and effective for genotyping DNA from fecal samples. Notably, the markers amplified and displayed microsatellite polymorphisms using DNA extracted from 11 fecal samples collected non-invasively from wild Sumatran rhinoceros. These microsatellite markers provide an important resource for a census and genetic studies of wild Sumatran rhinos.",
keywords = "Dicerorhinus sumatrensis, Non-invasive sampling, Short tandem repeats",
author = "Brandt, {Jessica R.} and Saidah, {Sinta H.} and Kai Zhao and Yasuko Ishida and Isabella Apriyana and Ryder, {Oliver A.} and Widodo Ramono and Herawati Sudoyo and Helena Suryadi and {Van Coeverden de Groot}, {Peter J.} and Roca, {Alfred L.}",
note = "Funding Information: For funding we thank the US Fish and Wildlife Service Rhinoceros and Tiger Conservation Fund (F15AP00758 and F18AP00853); the International Rhino Foundation; the Ministry of Research and Technology/National Research and Innovation Agency of the Republic of Indonesia; the World Wildlife Fund; the National Science and Engineering Research Council (Canada); and the UIUC ACES Office of International Program (Seed Grant and Graduate Student International Research Grant). JRB was supported by a UIUC Graduate College Dissertation Completion Fellowship. Funding Information: We thank the governments of Indonesia and Malaysia for enabling this research. We thank the Association and Zoos & Aquariums Rhino Advisory Group and the Rhino Research Council for enabling the collection of samples, and the USFWS inspectors at the Memphis Wildlife Inspection Office for guidance during the import process from Canada to the USA. For help in obtaining samples, we thank (in addition to co-authors) the Directorate General, Conservation of Natural Resources and Environment (KSDAE), Ministry of Environment and Forestry of the Republic of Indonesia; Indonesia Institute of Science; Burkit Barisan Selatan National Park Management Authority; Dr. Terri Roth (Cincinnati Zoo); Dr. Cynthia Steiner, Marlys Houck, Suellen Charter, and Julie Fronczek (San Diego Zoo ICR); Dr. Burton K. Lim and Jacqueline Miller (ROM). We thank the UIUC High-Throughput Sequencing and Genotyping Unit. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1186/s13104-021-05522-x",
language = "English (US)",
volume = "14",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "BioMed Central",
number = "1",
}