Characterization and purification of a phytotoxin produced by Fusarium solani, the causal agent of soybean sudden death syndrome

H. Jin, G. L. Hartman, C. D. Nickell, J. M. Widbolm

Research output: Contribution to journalArticle

Abstract

A phytotoxic polypeptide identified in culture filtrates of Fusarium solani, the causal agent of soybean sudden death syndrome, was heat unstable, negatively charged, absorbed by 10% charcoal, and destroyed by proteinase K. The toxicity of the culture filtrates and fractions obtained during purification was bioassayed by measuring browning of soybean calli. Purification of the phytotoxin was achieved by Sephadex G-50 gel filtration chromatography followed by ion exchange chromatography on a DE-52 column. The purified protain migrated as asingle band on sodium dodecyl sulfate-polyacrylamide gels with an estimated moleculer weight of 17,000. The sequence of the N-terminal 15 amino acids was determinated and indicated that a peptide was present. Samples containing this single protain caused browning of soybean calli, necrosis on detached soybeans cotyledons and leaves, and yellowing, curling, and drying of attached soybean cotyledons and leaves.

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalPhytopathology
Volume86
Issue number3
DOIs
StatePublished - Mar 1996

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Characterization and purification of a phytotoxin produced by Fusarium solani, the causal agent of soybean sudden death syndrome'. Together they form a unique fingerprint.

  • Cite this