TY - JOUR
T1 - Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06
AU - Chung, Chia Lin
AU - Jamann, Tiffany
AU - Longfellow, Joy
AU - Nelson, Rebecca
N1 - Funding Information:
We thank Elhan Ersoz for generous advice on fine-mapping, Kent Loeffler for photography, Jesse Poland and Judith Kolkman for helpful discussion, Major Goodman and Peter Balint-Kurti for providing the F seeds of S11 × DK888, and Pioneer Hi-Bred International Inc. for the supply of sorghum grains used in production of inoculum. We appreciate help from Ellie Walsh, Zura Kerdieh, Oliver Ott and Kristen Kennedy with aspects in the research. The work was funded by The CGIAR Generation Challenge Program, The McKnight Foundation, USDA-ARS, and Ministry of Education, Taiwan. 6
PY - 2010/7
Y1 - 2010/7
N2 - As part of a larger effort to capture diverse alleles at a set of loci associated with disease resistance in maize, DK888, a hybrid known to possess resistance to multiple diseases, was used as a donor in constructing near-isogenic lines (NILs). A NIL pair contrasting for resistance to northern leaf blight (NLB), caused by Setosphaeria turcica, was identified and associated with bin 8. 06. This region of the maize genome had been associated in previous studies with both qualitative and quantitative resistance to NLB. In addition, bins 8. 05-8. 06 had been associated with quantitative resistance to several other diseases, as well as resistance gene analogs and defense response gene homologs. To test the hypothesis that the DK888 allele at bin 8. 06 (designated qNLB8. 06DK888) conditions the broad-spectrum quantitative resistance characteristic of the donor, the NILs were evaluated with a range of maize pathogens and different races of S. turcica. The results revealed that qNLB8. 06DK888 confers race-specific resistance exclusively to NLB. Allelism analysis suggested that qNLB8. 06DK888 is identical, allelic, or closely linked and functionally related to Ht2. The resistance conditioned by qNLB8. 06 was incompletely dominant and varied in effectiveness depending upon allele and/or genetic background. High-resolution breakpoint analysis, using ~2,800 individuals in F9/F10 heterogeneous inbred families and 98 F10/F11 fixed lines carrying various recombinant events, delimited qNLB8. 06DK888 to a region of ~0. 46 Mb, spanning 143. 92-144. 38 Mb on the B73 physical map. Three compelling candidate genes were identified in this region. Isolation of the gene(s) will contribute to better understanding of this complex locus.
AB - As part of a larger effort to capture diverse alleles at a set of loci associated with disease resistance in maize, DK888, a hybrid known to possess resistance to multiple diseases, was used as a donor in constructing near-isogenic lines (NILs). A NIL pair contrasting for resistance to northern leaf blight (NLB), caused by Setosphaeria turcica, was identified and associated with bin 8. 06. This region of the maize genome had been associated in previous studies with both qualitative and quantitative resistance to NLB. In addition, bins 8. 05-8. 06 had been associated with quantitative resistance to several other diseases, as well as resistance gene analogs and defense response gene homologs. To test the hypothesis that the DK888 allele at bin 8. 06 (designated qNLB8. 06DK888) conditions the broad-spectrum quantitative resistance characteristic of the donor, the NILs were evaluated with a range of maize pathogens and different races of S. turcica. The results revealed that qNLB8. 06DK888 confers race-specific resistance exclusively to NLB. Allelism analysis suggested that qNLB8. 06DK888 is identical, allelic, or closely linked and functionally related to Ht2. The resistance conditioned by qNLB8. 06 was incompletely dominant and varied in effectiveness depending upon allele and/or genetic background. High-resolution breakpoint analysis, using ~2,800 individuals in F9/F10 heterogeneous inbred families and 98 F10/F11 fixed lines carrying various recombinant events, delimited qNLB8. 06DK888 to a region of ~0. 46 Mb, spanning 143. 92-144. 38 Mb on the B73 physical map. Three compelling candidate genes were identified in this region. Isolation of the gene(s) will contribute to better understanding of this complex locus.
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U2 - 10.1007/s00122-010-1303-z
DO - 10.1007/s00122-010-1303-z
M3 - Article
C2 - 20217383
AN - SCOPUS:77956808744
SN - 0040-5752
VL - 121
SP - 205
EP - 227
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 2
ER -