Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean [Canavalia ensiformis (L.) DC]

Melissa Pires-Alves, Maria Fátima Grossi-De-Sá, Grace B.S. Barcellos, Célia R. Carlini, Marcelo G. Moraes

Research output: Contribution to journalArticlepeer-review


Canavalia ensiformis (jackbean) seeds contain the proteins urease and canatoxin, a variant form of the jackbean urease. Here we have cloned a cDNA encoding another isoform of urease, called JBURE-II. This cDNA was obtained by RT-PCR using as template total RNA extracted from C. ensiformis tissues. Nucleotide sequence analysis showed that JBURE-II clones share 86% similarity with known jackbean urease. The presence in C. ensiformis of a family of urease-related genes with at least three members was demonstrated by Southern blot analysis. In order to understand the pattern of expression of the JBURE-II gene, we collected tissue samples from different stages of flower and embryo development. The results of RT-PCR show that JBURE-II is expressed from flower buds throughout seed maturation. Semi-quantitative RT-PCR indicates that expression of urease and JBURE-II genes is induced in seedlings and in leaves treated with abscisic acid, a phytohormone involved in seed maturation and wound response. This work constitutes the first report on the presence of a family of urease genes in jackbean, and provides characterization of a cDNA encoding a new member of this gene family.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalPlant and Cell Physiology
Issue number2
StatePublished - Feb 1 2003
Externally publishedYes


  • Abscisic acid
  • Canavalia ensiformis
  • Gene
  • Jackbean
  • Seed maturation
  • Urease (EC

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology


Dive into the research topics of 'Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean [Canavalia ensiformis (L.) DC]'. Together they form a unique fingerprint.

Cite this