TY - JOUR
T1 - Characterization and antimutagenic activity of soybean saponins
AU - Berhow, Mark A.
AU - Wagner, Elizabeth D.
AU - Vaughn, Steven F.
AU - Plewa, Michael J.
N1 - Funding Information:
This research was supported, in part, by United Soybean Board grant 7343. We wish to acknowledge the kind generosity of Dr. Eric Gugger and Archer Daniels Midland in providing soybean processing materials and products, and the technical assistance of Barry Jones, Kristen Naschansky, Susan Bartolini and Ray Holloway. The authors thank Dr. David Weisleder (USDA) for providing the NMR data.
PY - 2000/3/14
Y1 - 2000/3/14
N2 - An extract was prepared from a commercial soybean-processing by-product (soybean molasses) and was fractionated into purified chemical components. In previous work, this extract (phytochemical concentrate, PCC) repressed induced genomic DNA damage, whole cell clastogenicity and point mutation in cultured mammalian cells. In the current study, a chemical fraction was isolated from PCC using preparative high-performance liquid chromatography (HPLC). This fraction, PCC100, repressed 2-acetoxyacetylaminofluorene (2AAAF)-induced DNA damage in Chinese hamster ovary (CHO) cells as measured by single cell gel electrophoresis (alkaline Comet assay). Using liquid chromatography-electrospray ionization-mass spectroscopy and 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, PCC100 was shown to consist of a mixture of group B soyasaponins and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H- pyran-4-one (DDMP) soyasaponins. These include soyasaponins I, II, III, IV, V, Be, βg, βa, γg and γa. Purified soyasapogenol B aglycone prepared from fraction PCC100 demonstrated significant antigenotoxic activity against 2AAAF. To our knowledge, these data demonstrate for the first time the antimutagenic activity of soybean saponins in mammalian cells. (C) 2000 Elsevier Science B.V.
AB - An extract was prepared from a commercial soybean-processing by-product (soybean molasses) and was fractionated into purified chemical components. In previous work, this extract (phytochemical concentrate, PCC) repressed induced genomic DNA damage, whole cell clastogenicity and point mutation in cultured mammalian cells. In the current study, a chemical fraction was isolated from PCC using preparative high-performance liquid chromatography (HPLC). This fraction, PCC100, repressed 2-acetoxyacetylaminofluorene (2AAAF)-induced DNA damage in Chinese hamster ovary (CHO) cells as measured by single cell gel electrophoresis (alkaline Comet assay). Using liquid chromatography-electrospray ionization-mass spectroscopy and 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, PCC100 was shown to consist of a mixture of group B soyasaponins and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H- pyran-4-one (DDMP) soyasaponins. These include soyasaponins I, II, III, IV, V, Be, βg, βa, γg and γa. Purified soyasapogenol B aglycone prepared from fraction PCC100 demonstrated significant antigenotoxic activity against 2AAAF. To our knowledge, these data demonstrate for the first time the antimutagenic activity of soybean saponins in mammalian cells. (C) 2000 Elsevier Science B.V.
KW - Chemical fractionation
KW - Chemoprotectant
KW - Chinese hamster ovary cells
KW - Comet assay
KW - Phytochemical
KW - Sapogenol
KW - Saponin
KW - Single cell gel electrophoresis (SCGE)
KW - Soybean
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U2 - 10.1016/S0027-5107(99)00225-0
DO - 10.1016/S0027-5107(99)00225-0
M3 - Article
C2 - 10751618
AN - SCOPUS:0034646367
SN - 1386-1964
VL - 448
SP - 11
EP - 22
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1
ER -