Chapter 3: Microplate-based Comet Assay

Elizabeth D. Wagner, Michael J. Plewa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved. In this chapter we shall describe some of our work with a series of high-priority nitrogen-containing drinking-water disinfection byproducts (DBPs) that were synthesised as high-purity chemical analytical standards. The use of 96-well microplates allowed us to analyse the genotoxicity of these DBPs in mammalian cells.

Original languageEnglish (US)
Title of host publicationThe Comet Assay in Toxicology, 2nd Edition
EditorsDiana Anderson, Alok Dhawan
PublisherRoyal Society of Chemistry
Pages93-111
Number of pages19
Edition30
DOIs
StatePublished - 2017

Publication series

NameIssues in Toxicology
Number30
Volume2017-January
ISSN (Print)1757-7179
ISSN (Electronic)1757-7187

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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