Chapter 21 In Vitro Studies of Lantibiotic Biosynthesis

Bo Li, Lisa E. Cooper, Wilfred A. van der Donk

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The lantibiotics are ribosomally synthesized and posttranslationally modified peptide antibiotics containing the thioether crosslinks lanthionine (Lan) and 3-methyllanthionine (MeLan) and typically also the dehydroamino acids dehydroalanine (Dha) and (Z)-dehydrobutyrine (Dhb). These modifications are formed by dehydration of Ser/Thr residues to produce the Dha and Dhb structures, and subsequent conjugate additions of Cys residues onto the unsaturated amino acids to form thioether rings (Lan and MeLan). Several of the enzymatic reactions involved in lantibiotic biosynthesis have been reconstituted in vitro in recent years and these systems as well as a general overview of lantibiotic biosynthesis are discussed in this chapter.

Original languageEnglish (US)
Title of host publicationComplex Enzymes in Microbial Natural Product Biosynthesis, Part A
Subtitle of host publicationOverview Articles and Peptides
EditorsDavid Hopwood
Pages533-558
Number of pages26
EditionA
DOIs
StatePublished - Apr 16 2009

Publication series

NameMethods in Enzymology
NumberA
Volume458
ISSN (Print)0076-6879

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Li, B., Cooper, L. E., & van der Donk, W. A. (2009). Chapter 21 In Vitro Studies of Lantibiotic Biosynthesis. In D. Hopwood (Ed.), Complex Enzymes in Microbial Natural Product Biosynthesis, Part A: Overview Articles and Peptides (A ed., pp. 533-558). (Methods in Enzymology; Vol. 458, No. A). https://doi.org/10.1016/S0076-6879(09)04821-6