CHAPTER 2: Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity

C. Cui, J. H. Reed, E. N. Mirts, Yi Lu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

O2-dependent heme enzymes such as oxidases and oxygenases play important roles in numerous biological processes and much progress has been made in understanding structural features responsible for their diverse and efficient functions. An ultimate test of our knowledge in this area is to apply the insights gained from the study of native enzymes and their variants to design and engineer novel heme enzymes with similar O2-dependent catalytic activity. This endeavor will not only enrich our knowledge further, but also result in artificial catalysts for biomedical applications. In this chapter, we will highlight recent progress made in the design and engineering of structural and functional models of heme-containing monooxygenases, dioxygenases, and oxidases by redesigning native enzymes or by de novo design of new enzymes. In addition, heme-containing DNA or RNA enzymes with oxidase activity are also described. Finally, insights gained from these studies and perspectives for future directions are also summarized.

LanguageEnglish (US)
Title of host publicationDioxygen-dependent Heme Enzymes
EditorsMasao Ikeda-Saito, Emma Raven
PublisherRoyal Society of Chemistry
Pages37-62
Number of pages26
Edition13
DOIs
StatePublished - Jan 1 2019

Publication series

NameRSC Metallobiology
No.13
Volume2019-January
ISSN (Print)2045-547X

Fingerprint

Heme
Catalyst activity
Enzymes
Oxidoreductases
Biological Phenomena
Dioxygenases
Oxygenases
Structural Models
Mixed Function Oxygenases
RNA
Engineers
Catalysts
DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Cui, C., Reed, J. H., Mirts, E. N., & Lu, Y. (2019). CHAPTER 2: Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity. In M. Ikeda-Saito, & E. Raven (Eds.), Dioxygen-dependent Heme Enzymes (13 ed., pp. 37-62). (RSC Metallobiology; Vol. 2019-January, No. 13). Royal Society of Chemistry. https://doi.org/10.1039/9781788012911-00037

CHAPTER 2 : Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity. / Cui, C.; Reed, J. H.; Mirts, E. N.; Lu, Yi.

Dioxygen-dependent Heme Enzymes. ed. / Masao Ikeda-Saito; Emma Raven. 13. ed. Royal Society of Chemistry, 2019. p. 37-62 (RSC Metallobiology; Vol. 2019-January, No. 13).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cui, C, Reed, JH, Mirts, EN & Lu, Y 2019, CHAPTER 2: Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity. in M Ikeda-Saito & E Raven (eds), Dioxygen-dependent Heme Enzymes. 13 edn, RSC Metallobiology, no. 13, vol. 2019-January, Royal Society of Chemistry, pp. 37-62. https://doi.org/10.1039/9781788012911-00037
Cui C, Reed JH, Mirts EN, Lu Y. CHAPTER 2: Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity. In Ikeda-Saito M, Raven E, editors, Dioxygen-dependent Heme Enzymes. 13 ed. Royal Society of Chemistry. 2019. p. 37-62. (RSC Metallobiology; 13). https://doi.org/10.1039/9781788012911-00037
Cui, C. ; Reed, J. H. ; Mirts, E. N. ; Lu, Yi. / CHAPTER 2 : Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity. Dioxygen-dependent Heme Enzymes. editor / Masao Ikeda-Saito ; Emma Raven. 13. ed. Royal Society of Chemistry, 2019. pp. 37-62 (RSC Metallobiology; 13).
@inbook{6db3ac1a41f94945a913ccb81cb47e55,
title = "CHAPTER 2: Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity",
abstract = "O2-dependent heme enzymes such as oxidases and oxygenases play important roles in numerous biological processes and much progress has been made in understanding structural features responsible for their diverse and efficient functions. An ultimate test of our knowledge in this area is to apply the insights gained from the study of native enzymes and their variants to design and engineer novel heme enzymes with similar O2-dependent catalytic activity. This endeavor will not only enrich our knowledge further, but also result in artificial catalysts for biomedical applications. In this chapter, we will highlight recent progress made in the design and engineering of structural and functional models of heme-containing monooxygenases, dioxygenases, and oxidases by redesigning native enzymes or by de novo design of new enzymes. In addition, heme-containing DNA or RNA enzymes with oxidase activity are also described. Finally, insights gained from these studies and perspectives for future directions are also summarized.",
author = "C. Cui and Reed, {J. H.} and Mirts, {E. N.} and Yi Lu",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/9781788012911-00037",
language = "English (US)",
series = "RSC Metallobiology",
publisher = "Royal Society of Chemistry",
number = "13",
pages = "37--62",
editor = "Masao Ikeda-Saito and Emma Raven",
booktitle = "Dioxygen-dependent Heme Enzymes",
edition = "13",

}

TY - CHAP

T1 - CHAPTER 2

T2 - Design and Engineering of Heme Enzymes with O2-dependent Catalytic Activity

AU - Cui, C.

AU - Reed, J. H.

AU - Mirts, E. N.

AU - Lu, Yi

PY - 2019/1/1

Y1 - 2019/1/1

N2 - O2-dependent heme enzymes such as oxidases and oxygenases play important roles in numerous biological processes and much progress has been made in understanding structural features responsible for their diverse and efficient functions. An ultimate test of our knowledge in this area is to apply the insights gained from the study of native enzymes and their variants to design and engineer novel heme enzymes with similar O2-dependent catalytic activity. This endeavor will not only enrich our knowledge further, but also result in artificial catalysts for biomedical applications. In this chapter, we will highlight recent progress made in the design and engineering of structural and functional models of heme-containing monooxygenases, dioxygenases, and oxidases by redesigning native enzymes or by de novo design of new enzymes. In addition, heme-containing DNA or RNA enzymes with oxidase activity are also described. Finally, insights gained from these studies and perspectives for future directions are also summarized.

AB - O2-dependent heme enzymes such as oxidases and oxygenases play important roles in numerous biological processes and much progress has been made in understanding structural features responsible for their diverse and efficient functions. An ultimate test of our knowledge in this area is to apply the insights gained from the study of native enzymes and their variants to design and engineer novel heme enzymes with similar O2-dependent catalytic activity. This endeavor will not only enrich our knowledge further, but also result in artificial catalysts for biomedical applications. In this chapter, we will highlight recent progress made in the design and engineering of structural and functional models of heme-containing monooxygenases, dioxygenases, and oxidases by redesigning native enzymes or by de novo design of new enzymes. In addition, heme-containing DNA or RNA enzymes with oxidase activity are also described. Finally, insights gained from these studies and perspectives for future directions are also summarized.

UR - http://www.scopus.com/inward/record.url?scp=85056588401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056588401&partnerID=8YFLogxK

U2 - 10.1039/9781788012911-00037

DO - 10.1039/9781788012911-00037

M3 - Chapter

T3 - RSC Metallobiology

SP - 37

EP - 62

BT - Dioxygen-dependent Heme Enzymes

A2 - Ikeda-Saito, Masao

A2 - Raven, Emma

PB - Royal Society of Chemistry

ER -