Changes in Radioimmunoassayable Luteinizing Hormone-Releasing Hormone in Discrete Brain Areas of the Rat at Various Times on Proestrus, Diestrous Day 1, and after Phenobarbital Administration

Phyllis M Wise, Naomi Rance, Michael Selmanoff, Charles A. Barraclough

    Research output: Contribution to journalArticle

    Abstract

    Specific areas of the preoptico-suprachiasmatictuberoinfundibular system (PSTS) were microdissected at various hours on proestrus and diestrous day 1 in rats. LHRH concentrations were measured by RIA to determine if the patterns of change observed in the median eminence (ME) were paralleled by similar LHRH patterns in the remainder of the PSTS. Another group of proestrous rats received phenobarbital to block preovulatory gonadotropin surges, and LHRH concentrations in the PSTS were measured throughout the afternoon hours. Plasma concentrations of LH, FSH, estradiol, and progesterone were determined in all animals. Rats were decapitated on proestrus and diestrous day 1 at 0900, 1200, 1500, and 1800 h. Proestrous animals that received phenobarbital at noon were sacrificed at 1500 and 1800 h. The following brain areas were microdissected: the suprachiasmatic preoptic nucleus, medial preoptic nucleus, suprachiasmatic nucleus, anterior hypothalamic nucleus retrochiasmatic area, arcuate nucleus, and ME. On the day of proestrus, LHRH concentrations increased between 0900–1200 h (before the gonadotropin surge), decreased between 1200–1500 h (during the surge) and remained low at 1800 h in the medial preoptic nucleus, suprachiasmatic nucleus, retrochiasmatic area, and median eminence. A similar trend was observed in the anterior hypothalamic nucleus and suprachiasmatic preoptic nucleus, but only the drop in the suprachiasmatic preoptic nucleus, between 1200–1500 h attained statistical significance. No such patterns of change were observed on diestrous day 1. Phenobarbital blocked the LH and FSH surges but failed to prevent the afternoon decline in LHRH between 1200-1500 h in any of the brain regions examined. The morning rise and afternoon fall in LHRH may represent an increase in the synthesis and storage of the hormone which is released during the afternoon hours. The PSTS appears to function as an integrated unit, the component parts of which may be involved in synthesis, transport, storage, and the ultimate release of LHRH on proestrus.

    Original languageEnglish (US)
    Pages (from-to)2179-2185
    Number of pages7
    JournalEndocrinology
    Volume108
    Issue number6
    DOIs
    StatePublished - Jun 1981

    Fingerprint

    Proestrus
    Phenobarbital
    Gonadotropin-Releasing Hormone
    Suprachiasmatic Nucleus
    Preoptic Area
    Brain
    Median Eminence
    Anterior Hypothalamic Nucleus
    Gonadotropins
    Arcuate Nucleus of Hypothalamus
    Progesterone
    Estradiol
    Hormones

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Changes in Radioimmunoassayable Luteinizing Hormone-Releasing Hormone in Discrete Brain Areas of the Rat at Various Times on Proestrus, Diestrous Day 1, and after Phenobarbital Administration. / Wise, Phyllis M; Rance, Naomi; Selmanoff, Michael; Barraclough, Charles A.

    In: Endocrinology, Vol. 108, No. 6, 06.1981, p. 2179-2185.

    Research output: Contribution to journalArticle

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    abstract = "Specific areas of the preoptico-suprachiasmatictuberoinfundibular system (PSTS) were microdissected at various hours on proestrus and diestrous day 1 in rats. LHRH concentrations were measured by RIA to determine if the patterns of change observed in the median eminence (ME) were paralleled by similar LHRH patterns in the remainder of the PSTS. Another group of proestrous rats received phenobarbital to block preovulatory gonadotropin surges, and LHRH concentrations in the PSTS were measured throughout the afternoon hours. Plasma concentrations of LH, FSH, estradiol, and progesterone were determined in all animals. Rats were decapitated on proestrus and diestrous day 1 at 0900, 1200, 1500, and 1800 h. Proestrous animals that received phenobarbital at noon were sacrificed at 1500 and 1800 h. The following brain areas were microdissected: the suprachiasmatic preoptic nucleus, medial preoptic nucleus, suprachiasmatic nucleus, anterior hypothalamic nucleus retrochiasmatic area, arcuate nucleus, and ME. On the day of proestrus, LHRH concentrations increased between 0900–1200 h (before the gonadotropin surge), decreased between 1200–1500 h (during the surge) and remained low at 1800 h in the medial preoptic nucleus, suprachiasmatic nucleus, retrochiasmatic area, and median eminence. A similar trend was observed in the anterior hypothalamic nucleus and suprachiasmatic preoptic nucleus, but only the drop in the suprachiasmatic preoptic nucleus, between 1200–1500 h attained statistical significance. No such patterns of change were observed on diestrous day 1. Phenobarbital blocked the LH and FSH surges but failed to prevent the afternoon decline in LHRH between 1200-1500 h in any of the brain regions examined. The morning rise and afternoon fall in LHRH may represent an increase in the synthesis and storage of the hormone which is released during the afternoon hours. The PSTS appears to function as an integrated unit, the component parts of which may be involved in synthesis, transport, storage, and the ultimate release of LHRH on proestrus.",
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