Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities

Xiaoyu Liu, Daniel P. Nemeth, Daniel B. McKim, Ling Zhu, Damon J. DiSabato, Olimpia Berdysz, Gowthami Gorantla, Braedan Oliver, Kristina G. Witcher, Yufen Wang, Christina E. Negray, Rekha S. Vegesna, John F. Sheridan, Jonathan P. Godbout, Matthew J. Robson, Randy D. Blakely, Phillip G. Popovich, Staci D. Bilbo, Ning Quan

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-1 (IL-1) signaling is important for multiple potentially pathogenic processes in the central nervous system (CNS), but the cell-type-specific roles of IL-1 signaling are unclear. We used a genetic knockin reporter system in mice to track and reciprocally delete or express IL-1 receptor 1 (IL-1R1) in specific cell types, including endothelial cells, ventricular cells, peripheral myeloid cells, microglia, astrocytes, and neurons. We found that endothelial IL-1R1 was necessary and sufficient for mediating sickness behavior and drove leukocyte recruitment to the CNS and impaired neurogenesis, whereas ventricular IL-1R1 was critical for monocyte recruitment to the CNS. Although microglia did not express IL-1R1, IL-1 stimulation of endothelial cells led to the induction of IL-1 in microglia. Together, these findings describe the structure and functions of the brain's IL-1R1-expressing system and lay a foundation for the dissection and identification of IL-1R1 signaling pathways in the pathogenesis of CNS diseases. Liu et al. employ a genetic knockin reporter system in mice to track and reciprocally delete and/or express IL-1 receptor 1 (IL-1R1) in specific CNS cell types. They define cell-type-specific roles for IL-1 signaling, including an essential role for endothelial IL-1R1 in mediating sickness behavior, and provide a foundation for the dissection of IL-1R1 signaling pathways in the pathogenesis of CNS disease.

Original languageEnglish (US)
Pages (from-to)317-333.e6
JournalImmunity
Volume50
Issue number2
DOIs
StatePublished - Feb 19 2019

Keywords

  • leukocytes
  • microglia
  • neurogenesis
  • ventricular

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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