Abstract
Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 839-844 |
| Number of pages | 6 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 145 |
| DOIs | |
| State | Published - Sep 1 2016 |
Keywords
- 3T3
- Biomaterials
- Cell viability
- Spreading
- Tissue transglutaminase
- Zein
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
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Cui, H., Liu, G. L., & Padua, G. W. (2016). Cell spreading and viability on zein films may be facilitated by transglutaminase. Colloids and Surfaces B: Biointerfaces, 145, 839-844. https://doi.org/10.1016/j.colsurfb.2016.05.048