Cell spreading and viability on zein films may be facilitated by transglutaminase

Hemiao Cui; Gang L. Liu; Graciela W. Padua

doi:10.1016/j.colsurfb.2016.05.048

Cell spreading and viability on zein films may be facilitated by transglutaminase

Hemiao Cui, Gang L. Liu, Graciela W. Padua

Research output: Contribution to journal › Article › peer-review

Abstract

Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.

Original language	English (US)
Pages (from-to)	839-844
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	145
DOIs	https://doi.org/10.1016/j.colsurfb.2016.05.048
State	Published - Sep 1 2016

Keywords

3T3
Biomaterials
Cell viability
Spreading
Tissue transglutaminase
Zein

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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title = "Cell spreading and viability on zein films may be facilitated by transglutaminase",

abstract = "Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.",

keywords = "3T3, Biomaterials, Cell viability, Spreading, Tissue transglutaminase, Zein",

author = "Hemiao Cui and Liu, {Gang L.} and Padua, {Graciela W.}",

note = "Funding Information: This work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1007301. Thanks go to Dr. Yue Zhuo, from Micro and Nano Technology Laboratory, UIUC, for helpful suggestions on cell culture experiments. The experiments were carried out in part in the Frederick Seitz Materials Research Laboratory Central Research Facilities, University of Illinois. ",

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AU - Cui, Hemiao

AU - Liu, Gang L.

AU - Padua, Graciela W.

N1 - Funding Information: This work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1007301. Thanks go to Dr. Yue Zhuo, from Micro and Nano Technology Laboratory, UIUC, for helpful suggestions on cell culture experiments. The experiments were carried out in part in the Frederick Seitz Materials Research Laboratory Central Research Facilities, University of Illinois.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.

AB - Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.

KW - 3T3

KW - Biomaterials

KW - Cell viability

KW - Spreading

KW - Tissue transglutaminase

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