Abstract

This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated up the gradients, independent of the steepness of the gradients used in this study. At the same local laminin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.

Original languageEnglish (US)
Pages (from-to)4250-4258
Number of pages9
JournalLangmuir
Volume22
Issue number9
DOIs
StatePublished - Apr 25 2006

Fingerprint

Extracellular Matrix Proteins
Laminin
polarity
proteins
Proteins
gradients
matrices
cells
Microfluidics
Rats
slopes
microfluidic devices
profiles
rats

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Cell migration and polarity on microfabricated gradients of extracellular matrix proteins. / Gunawan, Rico C.; Silvestre, Jonathan; Gaskins, H. Rex; Kenis, Paul J.A.; Leckband, Deborah E.

In: Langmuir, Vol. 22, No. 9, 25.04.2006, p. 4250-4258.

Research output: Contribution to journalArticle

@article{76e71e2ef96245afa34b7cad849921dc,
title = "Cell migration and polarity on microfabricated gradients of extracellular matrix proteins",
abstract = "This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated up the gradients, independent of the steepness of the gradients used in this study. At the same local laminin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.",
author = "Gunawan, {Rico C.} and Jonathan Silvestre and Gaskins, {H. Rex} and Kenis, {Paul J.A.} and Leckband, {Deborah E.}",
year = "2006",
month = "4",
day = "25",
doi = "10.1021/la0531493",
language = "English (US)",
volume = "22",
pages = "4250--4258",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Cell migration and polarity on microfabricated gradients of extracellular matrix proteins

AU - Gunawan, Rico C.

AU - Silvestre, Jonathan

AU - Gaskins, H. Rex

AU - Kenis, Paul J.A.

AU - Leckband, Deborah E.

PY - 2006/4/25

Y1 - 2006/4/25

N2 - This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated up the gradients, independent of the steepness of the gradients used in this study. At the same local laminin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.

AB - This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated up the gradients, independent of the steepness of the gradients used in this study. At the same local laminin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.

UR - http://www.scopus.com/inward/record.url?scp=33646420095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646420095&partnerID=8YFLogxK

U2 - 10.1021/la0531493

DO - 10.1021/la0531493

M3 - Article

C2 - 16618172

AN - SCOPUS:33646420095

VL - 22

SP - 4250

EP - 4258

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 9

ER -