Cell loss in integrated microfluidic device

Liang Zhu, Xue Li Peh, Hong Miao Ji, Cheng Yong Teo, Han Hua Feng, Wen Tso Liu

Research output: Contribution to journalArticlepeer-review


Cell loss during sample transporting from macro-components to micro-components in integrated microfluidic devices can considerably deteriorate cell detection sensitivity. This intrinsic cell loss was studied and effectively minimized through (a) increasing the tubing diameter connecting the sample storage and the micro-device, (b) applying a hydrodynamic focusing approach for sample delivering to reduce cells contacting and adhesion on the walls of micro-channel and chip inlet; (c) optimizing the filter design with a zigzag arrangement of pillars (13 μm in chamber depth and 0.8 μm in gap) to prolong the effective filter length, and iv) the use of diamond shaped pillar instead of normally used rectangular shape to reduce the gap length between any two given pillar (i.e. pressure drop) at the filter region. Cell trapping and immunofluorescent detection of 12 Giardia lamblia and 12 Cryptosporidium parvumcells in 150 μl solution and 50 MCF-7 breast cancer cells in 150 μl solution was completed within 15 min with trapping efficiencies improved from 79±11%, 50.8±5.5% and 41.3±3.6% without hydrodynamic focusing, respectively, to 90.8±5.8%, 89.8±16.6% and 77.0±9.2% with hydrodynamic focusing.

Original languageEnglish (US)
Pages (from-to)745-750
Number of pages6
JournalBiomedical microdevices
Issue number5
StatePublished - Oct 2007
Externally publishedYes


  • Cell loss
  • Hydrodynamic focusing
  • Immnunofluorescent assay
  • Lab-on-a-chip
  • Microfluidic device
  • Trapping efficiency

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology


Dive into the research topics of 'Cell loss in integrated microfluidic device'. Together they form a unique fingerprint.

Cite this