Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

Samantha G. Zambuto, Ishita Jain, Hannah S. Theriault, Gregory H. Underhill, Brendan A.C. Harley

Research output: Contribution to journalArticlepeer-review


Chirality is an intrinsic cellular property that describes cell polarization biases along the left–right axis, apicobasal axis, or front–rear axes. Cell chirality plays a significant role in the arrangement of organs in the body as well as in the orientation of organelles, cytoskeletons, and cells. Vascular networks within the endometrium, the mucosal inner lining of the uterus, commonly display spiral architectures that rapidly form across the menstrual cycle. Herein, the role of endometrial-relevant extracellular matrix stiffness, composition, and soluble signals on endometrial endothelial cell chirality is systematically examined using a high-throughput microarray. Endometrial endothelial cells display marked patterns of chirality as individual cells and as cohorts in response to substrate stiffness and environmental cues. Vascular networks formed from endometrial endothelial cells also display shifts in chirality as a function of exogenous hormones. Changes in cellular-scale chirality correlate with changes in vascular network parameters, suggesting a critical role for cellular chirality in directing endometrial vessel network organization.

Original languageEnglish (US)
Article number2303928
JournalAdvanced Healthcare Materials
Issue number12
Early online dateJan 30 2024
StatePublished - May 7 2024


  • biomaterials
  • chirality
  • endometrium
  • endothelial cells
  • micropatterning

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science


Dive into the research topics of 'Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells'. Together they form a unique fingerprint.

Cite this