Cell Biology: Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement?

Comert Kural, Hwajin Kim, Sheyum Syed, Gohta Goshima, Vladimir I. Gelfand, Paul R Selvin

Research output: Contribution to journalArticle

Abstract

We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.

Original languageEnglish (US)
Pages (from-to)1469-1472
Number of pages4
JournalScience
Volume308
Issue number5727
DOIs
StatePublished - Jun 3 2005

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Dyneins
Kinesin
Peroxisomes
Cell Biology
Cytoplasmic Dyneins
Optical Imaging
Organelles
Drosophila
Fluorescence
Warfare
Proteins

ASJC Scopus subject areas

  • General

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Cell Biology : Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement? / Kural, Comert; Kim, Hwajin; Syed, Sheyum; Goshima, Gohta; Gelfand, Vladimir I.; Selvin, Paul R.

In: Science, Vol. 308, No. 5727, 03.06.2005, p. 1469-1472.

Research output: Contribution to journalArticle

Kural, Comert ; Kim, Hwajin ; Syed, Sheyum ; Goshima, Gohta ; Gelfand, Vladimir I. ; Selvin, Paul R. / Cell Biology : Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement?. In: Science. 2005 ; Vol. 308, No. 5727. pp. 1469-1472.
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