Cationic, helical polypeptide-based gene delivery for IMR-90 fibroblasts and human embryonic stem cells

Jonathan Yen, Yanfeng Zhang, Nathan P. Gabrielson, Lichen Yin, Linna Guan, Isthier Chaudhury, Hua Lu, Fei Wang, Jianjun Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Diblock copolymers consisting of poly(ethylene glycol)-block-poly(γ- 4-(((2-(piperidin-1-yl)ethyl)amino)methyl)benzyl-l-glutamate) (PEG-b-PVBLG-8) were synthesized and evaluated for their ability to mediate gene delivery in hard-to-transfect cells like IMR-90 human fetal lung fibroblasts and human embryonic stem cells (hESCs). The PEG-b-PVBLG-8 contained a membrane-disruptive, cationic, helical polypeptide block (PVBLG-8) for complexing with DNA and a hydrophilic PEG block to improve the biocompatibility of the gene delivery vehicle. The incorporation of PEG effectively reduced the toxicity of the helical PVBLG-8 block without dramatically compromising the polymer's ability to destabilize membranes or form complexes with DNA. PEG-b-PVBLG-8 copolymers with low (n = 76) and high (n = 287) degrees of polymerization (n) of the PVBLG-8 block were synthesized and evaluated for gene delivery. PEG-b-PVBLG-8 diblock polymers with a high degree of polymerization have a greater transfection efficiency and lower toxicity in IMR-90 cells than the commercial reagent Lipofectamine 2000. The usefulness of PEG-b-PVBLG-8 was further demonstrated via the successful transfection of hESCs without a measured loss in cell pluripotency markers. This journal is

Original languageEnglish (US)
Pages (from-to)719-727
Number of pages9
JournalBiomaterials Science
Volume1
Issue number7
DOIs
StatePublished - Jul 2013

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)

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