Ca2+ activated and pH sensitive cyclic AMP phosphodiesterase in the nervous system of the mollusc Pleurobranchaea

Roger D. Calhoon, Rhanor Gillette

Research output: Contribution to journalArticle

Abstract

Regulation of cyclic AMP through its synthesis is known to be important in modulating the activity of molluscan neurons; however, no data exists regarding the regulation of cyclic AMP degradation. We find that cyclic AMP phosphodiesterase (PDE) activity in homogenates of the nervous system of the mollusc Pleurobranchea is significantly stimulated by calcium ion. Ca2+ stimulation is suppressed by the calmodulin antagonist trifluoperazine (TFP), indicating resemblance to the Ca2+-calmodulin PDEs of mammalian neurons. Ca2+ also accentuates the pH sensitivity of PDE. The qualities of Ca2+ and pH sensitivity of PDE are fitted into a model for cAMP regulation of neuronal activity in an identified feeding command neuron; the postulated role of PDE is consistent with effects of cAMP, TFP, and pH on the neuron's activity.

Original languageEnglish (US)
Pages (from-to)371-374
Number of pages4
JournalBrain Research
Volume271
Issue number2
DOIs
StatePublished - Jul 25 1983

Fingerprint

Pleurobranchaea
Mollusca
Phosphoric Diester Hydrolases
Cyclic AMP
Nervous System
Neurons
Trifluoperazine
Calmodulin
Ions
Calcium

Keywords

  • Pleurobranchaea
  • calmodulin
  • cyclic AMP
  • intracellular pH
  • phenothiazine
  • phosphodiesterase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Ca2+ activated and pH sensitive cyclic AMP phosphodiesterase in the nervous system of the mollusc Pleurobranchaea. / Calhoon, Roger D.; Gillette, Rhanor.

In: Brain Research, Vol. 271, No. 2, 25.07.1983, p. 371-374.

Research output: Contribution to journalArticle

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