Cassettes for seed-specific expression tested in transformed embryogenic cultures of soybean

Myeong Je Cho, Jack M. Widholm, Lila O. Vodkin

Research output: Contribution to journalArticle

Abstract

Soybean (Glycine max [L.] Merrill) lectin is a seed protein that accumulates in protein bodies of cotyledons during seed development. We have constructed two expression cassettes containing the 5′ and 3′ regions of the soybean lectin gene connected by a Not I restriction site. One vector also contains the 32 amino acid signal sequence. Using polymerase chain reaction (PCR), the coding region of the β-glucuronidase (uidA) gene was inserted into the Not I site of each vector. We tested the function of the expression cassettes in transformed embryogenic cultures of soybean. Development-specific GUS expression was observed in developing somatic embryos transformed with the chimeric lectin promoter-GUS constructs as determined by histochemical assays. Our data indicate that these cassettes could be used to drive expression of foreign genes to modify embryo-specific traits of soybean as protein quality or quantity in the seed.

Original languageEnglish (US)
Pages (from-to)255-269
Number of pages15
JournalPlant Molecular Biology Reporter
Volume13
Issue number3
DOIs
StatePublished - Sep 1 1995

Keywords

  • embryogenic cultures
  • lectin promoter
  • seed specific expression
  • soybean

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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