Casein Inhibition of Clostridium perfringens Growth and Exoprotease Production

JOANNE M. CURRAN, MYRON SOLBERG, Hans-Peter M Blaschek, DAVID ROSEN

Research output: Contribution to journalArticle

Abstract

Growth of C. perfringens strain 3624 on a defined medium containing ANRC Reference Casein as the nitrogen source was investigated. Gas production, monitored manometrically as an index of growth, indicated a depressed growth response of the organism in the casein‐containing medium. Increased growth response resulting from pepsin treatment of the casein was largely due to the enzyme serving as a nitrogen source. Chelation of iron by casein was not responsible for the growth inhibition. Casein suspended in complete medium was not inhibitory to the organism. Growth studies indicated that native casein was not available as a nitrogen source to the organism. Acid hydrolyzed casein and commercial casein hydrolysate served as suitable nitrogen sources for growth of the organism, however exoprotease production was repressed in both media as well as in medium containing native casein as the sole nitrogen source. C. perfringens strain 3624 produced exoprotease in the completely defined medium containing synthetic amino acids, indicating the possible presence of an amino acid or a metabolite, not produced in the casein‐containing medium which functioned to derepress the enzyme synthesizing mechanism.

Original languageEnglish (US)
Pages (from-to)169-177
Number of pages9
JournalJournal of food science
Volume46
Issue number1
DOIs
StatePublished - Jan 1981

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Exopeptidases
Clostridium perfringens
Caseins
casein
Nitrogen
Growth
nitrogen
organisms
Amino Acids
casein hydrolysates
amino acids
gas production (biological)
chelation
Pepsin A
Enzymes
pepsin
enzymes
growth retardation
Iron
Gases

ASJC Scopus subject areas

  • Food Science

Cite this

Casein Inhibition of Clostridium perfringens Growth and Exoprotease Production. / CURRAN, JOANNE M.; SOLBERG, MYRON; Blaschek, Hans-Peter M; ROSEN, DAVID.

In: Journal of food science, Vol. 46, No. 1, 01.1981, p. 169-177.

Research output: Contribution to journalArticle

CURRAN, JOANNE M. ; SOLBERG, MYRON ; Blaschek, Hans-Peter M ; ROSEN, DAVID. / Casein Inhibition of Clostridium perfringens Growth and Exoprotease Production. In: Journal of food science. 1981 ; Vol. 46, No. 1. pp. 169-177.
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