Cascading Effects of Nanoparticle Coatings: Surface Functionalization Dictates the Assemblage of Complexed Proteins and Subsequent Interaction with Model Cell Membranes

Eric S. Melby, Samuel E. Lohse, Ji Eun Park, Ariane M. Vartanian, Rebecca A. Putans, Hannah B. Abbott, Robert J. Hamers, Catherine J. Murphy, Joel A. Pedersen

Research output: Contribution to journalArticle

Abstract

Interactions of functionalized nanomaterials with biological membranes are expected to be governed by not only nanoparticle physiochemical properties but also coatings or "coronas" of biomacromolecules acquired after immersion in biological fluids. Here we prepared a library of 4-5 nm gold nanoparticles (AuNPs) coated with either ω-functionalized thiols or polyelectrolyte wrappings to examine the influence of surface functional groups on the assemblage of proteins complexing the nanoparticles and its subsequent impact on attachment to model biological membranes. We find that the initial nanoparticle surface coating has a cascading effect on interactions with model cell membranes by determining the assemblage of complexing proteins, which in turn influences subsequent interaction with model biological membranes. Each type of functionalized AuNP investigated formed complexes with a unique ensemble of serum proteins that depended on the initial surface coating of the nanoparticles. Formation of protein-nanoparticle complexes altered the electrokinetic, hydrodynamic, and plasmonic properties of the AuNPs. Complexation of the nanoparticles with proteins reduced the attachment of cationic AuNPs and promoted attachment of anionic AuNPs to supported lipid bilayers; this trend is observed with both lipid bilayers comprising 100% zwitterionic phospholipids and those incorporating anionic phosphatidylinositol. Complexation with serum proteins led to attachment of otherwise noninteracting oligo(ethylene glycol)-functionalized AuNPs to bilayers containing phosphatidylinositol. These results demonstrate the importance of considering both facets of the nano-bio interface: functional groups displayed on the nanoparticle surface and proteins complexing the nanoparticles influence interaction with biological membranes as does the molecular makeup of the membranes themselves.

Original languageEnglish (US)
Pages (from-to)5489-5499
Number of pages11
JournalACS Nano
Volume11
Issue number6
DOIs
StatePublished - Jun 27 2017

Fingerprint

Cell membranes
Nanoparticles
proteins
Proteins
coatings
Coatings
nanoparticles
Biological membranes
attachment
membranes
interactions
bionics
Lipid bilayers
Phosphatidylinositols
Complexation
serums
Functional groups
lipids
Blood Proteins
Ethylene Glycol

Keywords

  • gold nanoparticle
  • protein corona
  • supported lipid bilayer
  • surface chemistry

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Cascading Effects of Nanoparticle Coatings : Surface Functionalization Dictates the Assemblage of Complexed Proteins and Subsequent Interaction with Model Cell Membranes. / Melby, Eric S.; Lohse, Samuel E.; Park, Ji Eun; Vartanian, Ariane M.; Putans, Rebecca A.; Abbott, Hannah B.; Hamers, Robert J.; Murphy, Catherine J.; Pedersen, Joel A.

In: ACS Nano, Vol. 11, No. 6, 27.06.2017, p. 5489-5499.

Research output: Contribution to journalArticle

Melby, Eric S. ; Lohse, Samuel E. ; Park, Ji Eun ; Vartanian, Ariane M. ; Putans, Rebecca A. ; Abbott, Hannah B. ; Hamers, Robert J. ; Murphy, Catherine J. ; Pedersen, Joel A. / Cascading Effects of Nanoparticle Coatings : Surface Functionalization Dictates the Assemblage of Complexed Proteins and Subsequent Interaction with Model Cell Membranes. In: ACS Nano. 2017 ; Vol. 11, No. 6. pp. 5489-5499.
@article{5c683f6eea974486b286f339c6a0f085,
title = "Cascading Effects of Nanoparticle Coatings: Surface Functionalization Dictates the Assemblage of Complexed Proteins and Subsequent Interaction with Model Cell Membranes",
abstract = "Interactions of functionalized nanomaterials with biological membranes are expected to be governed by not only nanoparticle physiochemical properties but also coatings or {"}coronas{"} of biomacromolecules acquired after immersion in biological fluids. Here we prepared a library of 4-5 nm gold nanoparticles (AuNPs) coated with either ω-functionalized thiols or polyelectrolyte wrappings to examine the influence of surface functional groups on the assemblage of proteins complexing the nanoparticles and its subsequent impact on attachment to model biological membranes. We find that the initial nanoparticle surface coating has a cascading effect on interactions with model cell membranes by determining the assemblage of complexing proteins, which in turn influences subsequent interaction with model biological membranes. Each type of functionalized AuNP investigated formed complexes with a unique ensemble of serum proteins that depended on the initial surface coating of the nanoparticles. Formation of protein-nanoparticle complexes altered the electrokinetic, hydrodynamic, and plasmonic properties of the AuNPs. Complexation of the nanoparticles with proteins reduced the attachment of cationic AuNPs and promoted attachment of anionic AuNPs to supported lipid bilayers; this trend is observed with both lipid bilayers comprising 100{\%} zwitterionic phospholipids and those incorporating anionic phosphatidylinositol. Complexation with serum proteins led to attachment of otherwise noninteracting oligo(ethylene glycol)-functionalized AuNPs to bilayers containing phosphatidylinositol. These results demonstrate the importance of considering both facets of the nano-bio interface: functional groups displayed on the nanoparticle surface and proteins complexing the nanoparticles influence interaction with biological membranes as does the molecular makeup of the membranes themselves.",
keywords = "gold nanoparticle, protein corona, supported lipid bilayer, surface chemistry",
author = "Melby, {Eric S.} and Lohse, {Samuel E.} and Park, {Ji Eun} and Vartanian, {Ariane M.} and Putans, {Rebecca A.} and Abbott, {Hannah B.} and Hamers, {Robert J.} and Murphy, {Catherine J.} and Pedersen, {Joel A.}",
year = "2017",
month = "6",
day = "27",
doi = "10.1021/acsnano.7b00231",
language = "English (US)",
volume = "11",
pages = "5489--5499",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Cascading Effects of Nanoparticle Coatings

T2 - Surface Functionalization Dictates the Assemblage of Complexed Proteins and Subsequent Interaction with Model Cell Membranes

AU - Melby, Eric S.

AU - Lohse, Samuel E.

AU - Park, Ji Eun

AU - Vartanian, Ariane M.

AU - Putans, Rebecca A.

AU - Abbott, Hannah B.

AU - Hamers, Robert J.

AU - Murphy, Catherine J.

AU - Pedersen, Joel A.

PY - 2017/6/27

Y1 - 2017/6/27

N2 - Interactions of functionalized nanomaterials with biological membranes are expected to be governed by not only nanoparticle physiochemical properties but also coatings or "coronas" of biomacromolecules acquired after immersion in biological fluids. Here we prepared a library of 4-5 nm gold nanoparticles (AuNPs) coated with either ω-functionalized thiols or polyelectrolyte wrappings to examine the influence of surface functional groups on the assemblage of proteins complexing the nanoparticles and its subsequent impact on attachment to model biological membranes. We find that the initial nanoparticle surface coating has a cascading effect on interactions with model cell membranes by determining the assemblage of complexing proteins, which in turn influences subsequent interaction with model biological membranes. Each type of functionalized AuNP investigated formed complexes with a unique ensemble of serum proteins that depended on the initial surface coating of the nanoparticles. Formation of protein-nanoparticle complexes altered the electrokinetic, hydrodynamic, and plasmonic properties of the AuNPs. Complexation of the nanoparticles with proteins reduced the attachment of cationic AuNPs and promoted attachment of anionic AuNPs to supported lipid bilayers; this trend is observed with both lipid bilayers comprising 100% zwitterionic phospholipids and those incorporating anionic phosphatidylinositol. Complexation with serum proteins led to attachment of otherwise noninteracting oligo(ethylene glycol)-functionalized AuNPs to bilayers containing phosphatidylinositol. These results demonstrate the importance of considering both facets of the nano-bio interface: functional groups displayed on the nanoparticle surface and proteins complexing the nanoparticles influence interaction with biological membranes as does the molecular makeup of the membranes themselves.

AB - Interactions of functionalized nanomaterials with biological membranes are expected to be governed by not only nanoparticle physiochemical properties but also coatings or "coronas" of biomacromolecules acquired after immersion in biological fluids. Here we prepared a library of 4-5 nm gold nanoparticles (AuNPs) coated with either ω-functionalized thiols or polyelectrolyte wrappings to examine the influence of surface functional groups on the assemblage of proteins complexing the nanoparticles and its subsequent impact on attachment to model biological membranes. We find that the initial nanoparticle surface coating has a cascading effect on interactions with model cell membranes by determining the assemblage of complexing proteins, which in turn influences subsequent interaction with model biological membranes. Each type of functionalized AuNP investigated formed complexes with a unique ensemble of serum proteins that depended on the initial surface coating of the nanoparticles. Formation of protein-nanoparticle complexes altered the electrokinetic, hydrodynamic, and plasmonic properties of the AuNPs. Complexation of the nanoparticles with proteins reduced the attachment of cationic AuNPs and promoted attachment of anionic AuNPs to supported lipid bilayers; this trend is observed with both lipid bilayers comprising 100% zwitterionic phospholipids and those incorporating anionic phosphatidylinositol. Complexation with serum proteins led to attachment of otherwise noninteracting oligo(ethylene glycol)-functionalized AuNPs to bilayers containing phosphatidylinositol. These results demonstrate the importance of considering both facets of the nano-bio interface: functional groups displayed on the nanoparticle surface and proteins complexing the nanoparticles influence interaction with biological membranes as does the molecular makeup of the membranes themselves.

KW - gold nanoparticle

KW - protein corona

KW - supported lipid bilayer

KW - surface chemistry

UR - http://www.scopus.com/inward/record.url?scp=85021439754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021439754&partnerID=8YFLogxK

U2 - 10.1021/acsnano.7b00231

DO - 10.1021/acsnano.7b00231

M3 - Article

C2 - 28482159

AN - SCOPUS:85021439754

VL - 11

SP - 5489

EP - 5499

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 6

ER -