Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Keyphrases

• Biosynthetic Gene Clusters 100%
• Cre-mediated Recombination 100%
• Cas12a 100%
• Direct Cloning 75%
• Heterologous Expression 50%
• DNA Assembly 50%
• Cloning Method 50%
• Natural Products 25%
• Fill-in 25%
• Cre-loxP 25%
• Assembly Approach 25%
• Digestion 25%
• Bacillus Anthracis 25%
• Efficient Strategy 25%
• Polymerase 25%
• Gram-negative Bacteria 25%
• Effective Strategies 25%
• Gram-positive 25%
• Antimicrobial Activity 25%
• Carbon Skeleton 25%
• Bacillus 25%
• Pentacyclic 25%
• Methicillin-resistant Staphylococcus Aureus 25%
• Vancomycin-resistant Enterococci 25%
• Actinomycetes 25%
• Enterococcus Faecium 25%
• Circularization 25%
• High GC Content 25%
• Novel Bioactive Compounds 25%
• Bioweapons 25%

Biochemistry, Genetics and Molecular Biology

• Gene Cluster 100%
• Heterologous Expression 50%
• Polymerase 25%
• GC-content 25%
• Antimicrobial Activity 25%
• Bacilli 25%
• Gram Negative Bacterium 25%
• Methicillin Resistant Staphylococcus Aureus 25%
• Bacillus Anthracis 25%
• Enterococcus faecium 25%
• Actinobacteria 25%