Carnitine palmitoyltransferase modulation of hepatic fatty acid metabolism and radio-HPLC evidence for low ketogenesis in neonatal pigs

J. Odle, X. Lin, T. A.T.G. Van Kempen, J. K. Drackley, S. H. Adams

Research output: Contribution to journalArticlepeer-review

Abstract

A neonatal piglet model was used to study hepatic fatty acid metabolism during the early postnatal period. Hepatocytes were isolated from pigs at birth or after 24 h, in fed or unfed states (n = 4 pigs/group). Cells were incubated with 1 mmol/L [1-14C]-octanoate (C8) or -palmitate (C16) in the presence or absence of 1 mmol/L L-carnitine, carnitine plus tetradecylglycidic acid (TDGA; 10 μmol/L) or carnitine plus glucagon (0.5 μg/L). Accumulation of radiolabel [nmol/(h · 106 cells)] in CO2 and acid- soluble products (ASP) was higher (3.5- and 4.5-fold, respectively) from C8 than from C16 (P < 0.0001). Glucagon, carnitine and TDGA had no effect on the oxidation of C8 (P > 0.1). Carnitine addition tended to increase C16 flux to ASP [from 5.3 to 7.6 nmol/(h · 106 cells); P < 0.1], whereas carnitine plus TDGA decreased flux (from 7.6 to 2.1; P < 0.001). Esterified products accounted for 70% of metabolized label in control C16 incubations; this was reduced to 62% by carnitine (P < 0.05) and increased to 80% by the addition of carnitine plus TDGA (P < 0.0001). The 1-14C flux to CO2 in cells from 24-h-old unfed piglets was 47% lower than from fed pigs (P < 0.01) but 28% higher than in pigs at birth. Radiolabel contained in ASP and total metabolized label were 48% lower from unfed pigs compared with the piglets at birth and 24-h-old fed pigs (P < 0.01) and were paralleled by changes in oxygen consumption. Radio-HPLC analysis of ASP revealed minimal radiolabel accumulation in ketone bodies. Up to 40% of radioactivity in ASP was presumptively identified as acetate. These data illustrate that hepatic C16 metabolism in piglets can be altered by changes. In the activity of carnitine palmitoyltransferase 1 during the neonatal period and that ketone bodies do not represent a major route of hepatic fatty acid carbon flux.

Original languageEnglish (US)
Pages (from-to)2541-2549
Number of pages9
JournalJournal of Nutrition
Volume125
Issue number10
StatePublished - Jan 1 1995
Externally publishedYes

Keywords

  • fatty acid metabolism
  • hepatocyte
  • ketogenesis
  • neonate
  • swine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Carnitine palmitoyltransferase modulation of hepatic fatty acid metabolism and radio-HPLC evidence for low ketogenesis in neonatal pigs'. Together they form a unique fingerprint.

Cite this