Abstract

Spatial light interference microscopy (SLIM) is a highly sensitive quantitative phase imaging method, which is capable of unprecedented structure studies in biology and beyond. In addition to the π/2 shift introduced in phase contrast between the scattered and unscattered light from the sample, 4 phase shifts are generated in SLIM, by increments of π/2 using a reflective liquid crystal phase modulator (LCPM). As 4 phase shifted images are required to produce a quantitative phase image, the switching speed of the LCPM and the acquisition rate of the camera limit the acquisition rate and, thus, SLIM's applicability to highly dynamic samples. In this paper we present a fast SLIM setup which can image at a maximum rate of 50 frames per second and provide in real-time quantitative phase images at 50/4 = 12.5 frames per second. We use a fast LCPM for phase shifting and a fast scientific-grade complementary metal oxide semiconductor (sCMOS) camera (Andor) for imaging. We present the dispersion relation, i.e. decay rate vs. spatial mode, associated with dynamic beating cardiomyocyte cells from the quantitative phase images obtained with the real-time SLIM system.

Original languageEnglish (US)
Article numbere56930
JournalPloS one
Volume8
Issue number2
DOIs
StatePublished - Feb 15 2013

Fingerprint

Interference Microscopy
Light interference
crossover interference
Cardiac Myocytes
Liquid Crystals
microscopy
Microscopic examination
image analysis
Modulators
Imaging techniques
Light
cameras
Cameras
semiconductors
Semiconductors
Phase shift
Metals
Oxides
oxides
deterioration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Cardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM). / Bhaduri, Basanta; Wickland, David; Wang, Ru; Chan, Vincent; Bashir, Rashid; Popescu, Gabriel.

In: PloS one, Vol. 8, No. 2, e56930, 15.02.2013.

Research output: Contribution to journalArticle

Bhaduri, Basanta ; Wickland, David ; Wang, Ru ; Chan, Vincent ; Bashir, Rashid ; Popescu, Gabriel. / Cardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM). In: PloS one. 2013 ; Vol. 8, No. 2.
@article{6592051f3d7a4644a4fc01cb38c34d62,
title = "Cardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM)",
abstract = "Spatial light interference microscopy (SLIM) is a highly sensitive quantitative phase imaging method, which is capable of unprecedented structure studies in biology and beyond. In addition to the π/2 shift introduced in phase contrast between the scattered and unscattered light from the sample, 4 phase shifts are generated in SLIM, by increments of π/2 using a reflective liquid crystal phase modulator (LCPM). As 4 phase shifted images are required to produce a quantitative phase image, the switching speed of the LCPM and the acquisition rate of the camera limit the acquisition rate and, thus, SLIM's applicability to highly dynamic samples. In this paper we present a fast SLIM setup which can image at a maximum rate of 50 frames per second and provide in real-time quantitative phase images at 50/4 = 12.5 frames per second. We use a fast LCPM for phase shifting and a fast scientific-grade complementary metal oxide semiconductor (sCMOS) camera (Andor) for imaging. We present the dispersion relation, i.e. decay rate vs. spatial mode, associated with dynamic beating cardiomyocyte cells from the quantitative phase images obtained with the real-time SLIM system.",
author = "Basanta Bhaduri and David Wickland and Ru Wang and Vincent Chan and Rashid Bashir and Gabriel Popescu",
year = "2013",
month = "2",
day = "15",
doi = "10.1371/journal.pone.0056930",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Cardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM)

AU - Bhaduri, Basanta

AU - Wickland, David

AU - Wang, Ru

AU - Chan, Vincent

AU - Bashir, Rashid

AU - Popescu, Gabriel

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Spatial light interference microscopy (SLIM) is a highly sensitive quantitative phase imaging method, which is capable of unprecedented structure studies in biology and beyond. In addition to the π/2 shift introduced in phase contrast between the scattered and unscattered light from the sample, 4 phase shifts are generated in SLIM, by increments of π/2 using a reflective liquid crystal phase modulator (LCPM). As 4 phase shifted images are required to produce a quantitative phase image, the switching speed of the LCPM and the acquisition rate of the camera limit the acquisition rate and, thus, SLIM's applicability to highly dynamic samples. In this paper we present a fast SLIM setup which can image at a maximum rate of 50 frames per second and provide in real-time quantitative phase images at 50/4 = 12.5 frames per second. We use a fast LCPM for phase shifting and a fast scientific-grade complementary metal oxide semiconductor (sCMOS) camera (Andor) for imaging. We present the dispersion relation, i.e. decay rate vs. spatial mode, associated with dynamic beating cardiomyocyte cells from the quantitative phase images obtained with the real-time SLIM system.

AB - Spatial light interference microscopy (SLIM) is a highly sensitive quantitative phase imaging method, which is capable of unprecedented structure studies in biology and beyond. In addition to the π/2 shift introduced in phase contrast between the scattered and unscattered light from the sample, 4 phase shifts are generated in SLIM, by increments of π/2 using a reflective liquid crystal phase modulator (LCPM). As 4 phase shifted images are required to produce a quantitative phase image, the switching speed of the LCPM and the acquisition rate of the camera limit the acquisition rate and, thus, SLIM's applicability to highly dynamic samples. In this paper we present a fast SLIM setup which can image at a maximum rate of 50 frames per second and provide in real-time quantitative phase images at 50/4 = 12.5 frames per second. We use a fast LCPM for phase shifting and a fast scientific-grade complementary metal oxide semiconductor (sCMOS) camera (Andor) for imaging. We present the dispersion relation, i.e. decay rate vs. spatial mode, associated with dynamic beating cardiomyocyte cells from the quantitative phase images obtained with the real-time SLIM system.

UR - http://www.scopus.com/inward/record.url?scp=84874085124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874085124&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0056930

DO - 10.1371/journal.pone.0056930

M3 - Article

C2 - 23457641

AN - SCOPUS:84874085124

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e56930

ER -