Abstract

Cardiac myocytes originating from different parts of the heart exhibit varying morphology and ultrastructure. However, the difference in their dynamic behavior is unclear. We examined the contraction of cardiac myocytes originating from the apex, ventricle, and atrium, and found that their dynamic behavior, such as amplitude and frequency of contraction, differs depending on the heart segment of origin. Using video microscopy and high-precision image correlation, we found that: (1) apex myocytes exhibited the highest contraction rate (∼17beats/min); (2) ventricular myocytes exhibited the highest contraction amplitude (∼5.2micron); and (3) as myocyte contraction synchronized, their frequency did not change significantly, but the amplitude of contraction increased in apex and ventricular myocytes. In addition, as myocyte cultures mature they formed contractile filaments, further emphasizing the difference in myocyte dynamics is persistent. These results suggest that the dynamic behavior (in addition to static properties) of myocytes is dependent on their segment of origin.

Original languageEnglish (US)
Pages (from-to)628-636
Number of pages9
JournalBiotechnology and bioengineering
Volume110
Issue number2
DOIs
StatePublished - Feb 1 2013

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Cardiac Myocytes
Muscle Cells
Video Microscopy
Microscopic examination

Keywords

  • Cardiac tissue engineering
  • Cell transplantation
  • Heart physiology
  • Myocyte filament
  • Sarcomere length

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Cardiac myocytes' dynamic contractile behavior differs depending on heart segment. / De Souza, Emerson J.; Ahmed, Wylie; Chan, Vincent; Bashir, Rashid; Saif, Taher.

In: Biotechnology and bioengineering, Vol. 110, No. 2, 01.02.2013, p. 628-636.

Research output: Contribution to journalArticle

De Souza, Emerson J. ; Ahmed, Wylie ; Chan, Vincent ; Bashir, Rashid ; Saif, Taher. / Cardiac myocytes' dynamic contractile behavior differs depending on heart segment. In: Biotechnology and bioengineering. 2013 ; Vol. 110, No. 2. pp. 628-636.
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