Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA

Tapasree Roy Sarkar, Joseph Maria Kumar Irudayaraj

Research output: Contribution to journalArticle

Abstract

Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5′-NH2-tagged oligo-(dT)25 primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplification of β-actin to confirm the compatibility. These MNPs were also used to extract the sc form of pDNA from agarose gel. The compatibility of the pDNA was demonstrated by restriction digestion. Both of these methodologies are simple, inexpensive (compared with existing kits), and efficient.

Original languageEnglish (US)
Pages (from-to)130-132
Number of pages3
JournalAnalytical Biochemistry
Volume379
Issue number1
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

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Superhelical DNA
Nanoparticles
Plasmids
Sepharose
Messenger RNA
DNA
Gels
Cells
Amplification
Actins
Digestion
Breast Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA. / Sarkar, Tapasree Roy; Irudayaraj, Joseph Maria Kumar.

In: Analytical Biochemistry, Vol. 379, No. 1, 01.08.2008, p. 130-132.

Research output: Contribution to journalArticle

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