TY - JOUR
T1 - Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA
AU - Sarkar, Tapasree Roy
AU - Irudayaraj, Joseph
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5′-NH2-tagged oligo-(dT)25 primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplification of β-actin to confirm the compatibility. These MNPs were also used to extract the sc form of pDNA from agarose gel. The compatibility of the pDNA was demonstrated by restriction digestion. Both of these methodologies are simple, inexpensive (compared with existing kits), and efficient.
AB - Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5′-NH2-tagged oligo-(dT)25 primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplification of β-actin to confirm the compatibility. These MNPs were also used to extract the sc form of pDNA from agarose gel. The compatibility of the pDNA was demonstrated by restriction digestion. Both of these methodologies are simple, inexpensive (compared with existing kits), and efficient.
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U2 - 10.1016/j.ab.2008.04.016
DO - 10.1016/j.ab.2008.04.016
M3 - Article
C2 - 18471426
AN - SCOPUS:46549083665
SN - 0003-2697
VL - 379
SP - 130
EP - 132
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -