Carbohydrate storage in anaerobic sequencing batch reactors

Toshio Shimada; Julie Zilles; Lutgarde Raskin; Eberhard Morgenroth

doi:10.1016/j.watres.2007.06.052

Carbohydrate storage in anaerobic sequencing batch reactors

Toshio Shimada, Julie Zilles, Lutgarde Raskin, Eberhard Morgenroth

Research output: Contribution to journal › Article › peer-review

Abstract

This study demonstrates the accumulation and degradation of trehalose as a storage compound in a glucose-fed anaerobic sequencing batch reactor (ASBR). One hour after substrate addition, only 40% of the added organic matter (as chemical oxygen demand, COD) was accounted for by the cumulative methane production and soluble COD remaining in the reactor. All influent COD was accounted for by methane and biomass production by the end of the 24-h ASBR cycle. These dynamics can be explained by the production of an intracellular storage product. Total carbohydrate analysis showed that 26% of the glucose added to the reactor transiently accumulated within the biomass. Based on ¹³C-nuclear magnetic resonance (NMR) analysis, trehalose (α-d-glucopyranosyl-(d-glucopyranoside)) was identified as the main carbohydrate produced. Mathematical modeling was performed and the IWA Anaerobic Digestion Model No. 1 (ADM1) was modified to include microbial storage. The modified model adequately described the ASBR dynamics during a 24-h cycle.

Original language	English (US)
Pages (from-to)	4721-4729
Number of pages	9
Journal	Water Research
Volume	41
Issue number	20
DOIs	https://doi.org/10.1016/j.watres.2007.06.052
State	Published - Dec 2007

Keywords

ADM1
ASBR
Anaerobic Digestion Model No. 1
Anaerobic sequencing batch reactor
Microbial storage
Trehalose

ASJC Scopus subject areas

Ecological Modeling
Water Science and Technology
Waste Management and Disposal
Pollution

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title = "Carbohydrate storage in anaerobic sequencing batch reactors",

abstract = "This study demonstrates the accumulation and degradation of trehalose as a storage compound in a glucose-fed anaerobic sequencing batch reactor (ASBR). One hour after substrate addition, only 40% of the added organic matter (as chemical oxygen demand, COD) was accounted for by the cumulative methane production and soluble COD remaining in the reactor. All influent COD was accounted for by methane and biomass production by the end of the 24-h ASBR cycle. These dynamics can be explained by the production of an intracellular storage product. Total carbohydrate analysis showed that 26% of the glucose added to the reactor transiently accumulated within the biomass. Based on 13C-nuclear magnetic resonance (NMR) analysis, trehalose (α-d-glucopyranosyl-(d-glucopyranoside)) was identified as the main carbohydrate produced. Mathematical modeling was performed and the IWA Anaerobic Digestion Model No. 1 (ADM1) was modified to include microbial storage. The modified model adequately described the ASBR dynamics during a 24-h cycle.",

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author = "Toshio Shimada and Julie Zilles and Lutgarde Raskin and Eberhard Morgenroth",

note = "Funding Information: We appreciate the helpful discussions with Maria Reis about NMR applications and the assistance of the University of Illinois at Urbana-Champaign VOICE NMR Laboratory staff. Toshio Shimada was supported by a fellowship from the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACYT), Mexico. This research was partially supported by the US Department of Agriculture under Cooperative Agreement AG 58-3620-1-179.",

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AB - This study demonstrates the accumulation and degradation of trehalose as a storage compound in a glucose-fed anaerobic sequencing batch reactor (ASBR). One hour after substrate addition, only 40% of the added organic matter (as chemical oxygen demand, COD) was accounted for by the cumulative methane production and soluble COD remaining in the reactor. All influent COD was accounted for by methane and biomass production by the end of the 24-h ASBR cycle. These dynamics can be explained by the production of an intracellular storage product. Total carbohydrate analysis showed that 26% of the glucose added to the reactor transiently accumulated within the biomass. Based on 13C-nuclear magnetic resonance (NMR) analysis, trehalose (α-d-glucopyranosyl-(d-glucopyranoside)) was identified as the main carbohydrate produced. Mathematical modeling was performed and the IWA Anaerobic Digestion Model No. 1 (ADM1) was modified to include microbial storage. The modified model adequately described the ASBR dynamics during a 24-h cycle.

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Carbohydrate storage in anaerobic sequencing batch reactors

Abstract

Keywords

ASJC Scopus subject areas

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