Abstract
Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidimensional information often enables unambiguous identification of analytes. Combination of CE with information-rich wavelength-resolved fluorescence detection is analogous with ultraviolet-visible diode-array detection and furnishes both qualitative and quantitative chemical information about target species. This review discusses recent advances in wavelength-resolved laser-induced fluorescence detection coupled with CE, with an emphasis on instrument design.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 332-343 |
| Number of pages | 12 |
| Journal | Analytical and bioanalytical chemistry |
| Volume | 373 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2002 |
Keywords
- Capillary electrophoresis
- Laser-induced fluorescence
- Review
- Wavelength-resolved fluorescence
ASJC Scopus subject areas
- Clinical Biochemistry
- Analytical Chemistry