Capillary electrophoresis with wavelength-resolved laser-induced fluorescence detection

Xin Zhang, Jeffrey N. Stuart, Jonathan V Sweedler

Research output: Contribution to journalReview articlepeer-review

Abstract

Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidimensional information often enables unambiguous identification of analytes. Combination of CE with information-rich wavelength-resolved fluorescence detection is analogous with ultraviolet-visible diode-array detection and furnishes both qualitative and quantitative chemical information about target species. This review discusses recent advances in wavelength-resolved laser-induced fluorescence detection coupled with CE, with an emphasis on instrument design.

Original languageEnglish (US)
Pages (from-to)332-343
Number of pages12
JournalAnalytical and bioanalytical chemistry
Volume373
Issue number6
DOIs
StatePublished - Jan 1 2002

Keywords

  • Capillary electrophoresis
  • Laser-induced fluorescence
  • Review
  • Wavelength-resolved fluorescence

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

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