Cap-n-Collar promotes tissue regeneration by regulating ROS and JNK signaling in the drosophila melanogaster wing imaginal disc

Amanda R. Brock, Mabel Seto, Rachel Smith-Bolton

Research output: Contribution to journalArticle

Abstract

Regeneration is a complex process that requires an organism to recognize and repair tissue damage, as well as grow and pattern new tissue. Here, we describe a genetic screen to identify novel regulators of regeneration. We ablated the Drosophila melanogaster larval wing primordium by inducing apoptosis in a spatially and temporally controlled manner and allowed the tissue to regenerate and repattern. To identify genes that regulate regeneration, we carried out a dominant-modifier screen by assessing the amount and quality of regeneration in adult wings heterozygous for isogenic deficiencies. We have identified 31 regions on the right arm of the third chromosome that modify the regenerative response. Interestingly, we observed several distinct phenotypes: mutants that regenerated poorly, mutants that regenerated faster or better than wild-type, and mutants that regenerated imperfectly and had patterning defects. We mapped one deficiency region to cap-n-collar (cnc), the Drosophila Nrf2 ortholog, which is required for regeneration. Cnc regulates reactive oxygen species levels in the regenerating epithelium, and affects c-Jun N-terminal protein kinase (JNK) signaling, growth, debris localization, and pupariation timing. Here, we present the results of our screen and propose a model wherein Cnc regulates regeneration by maintaining an optimal level of reactive oxygen species to promote JNK signaling.

Original languageEnglish (US)
Pages (from-to)1505-1520
Number of pages16
JournalGenetics
Volume206
Issue number3
DOIs
StatePublished - Jul 2017

Fingerprint

Imaginal Discs
Drosophila melanogaster
Regeneration
Reactive Oxygen Species
JNK Mitogen-Activated Protein Kinases
Drosophila
protein kinase N
Epithelium
Chromosomes
Apoptosis
Phenotype
Growth
Genes

Keywords

  • Cap-n-collar
  • Drosophila
  • Imaginal disc
  • Nrf2
  • Reactive oxygen species
  • Regeneration

ASJC Scopus subject areas

  • Genetics

Cite this

Cap-n-Collar promotes tissue regeneration by regulating ROS and JNK signaling in the drosophila melanogaster wing imaginal disc. / Brock, Amanda R.; Seto, Mabel; Smith-Bolton, Rachel.

In: Genetics, Vol. 206, No. 3, 07.2017, p. 1505-1520.

Research output: Contribution to journalArticle

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