Can the exceptional chilling tolerance of C4 photosynthesis found in Miscanthus ;× giganteus be exceeded? Screening of a novel Miscanthus Japanese germplasm collection

Katarzyna Głowacka, Uffe Jørgensen, Jens B. Kjeldsen, Kirsten Kørup, Idan Spitz, Erik J. Sacks, Stephen P. Long

Research output: Contribution to journalArticle

Abstract

Background and Aims A clone of the hybrid perennial C4 grass Miscanthus ;× giganteus (Mxg) is known for achieving exceptionally high rates of leaf CO2 uptake during chilling. This is a requisite of success in the early spring, as is the ability of the leaves to survive occasional frosts. The aim of this study was to search for genotypes with greater potential than Mxg for photosynthesis and frost survival under these conditions. Methods A total of 864 accessions representing 164 local populations of M. sacchariflorus (Msa), M. sinensis (Msi) and M. tinctorius (Mti) collected across Japan were studied. Accessions whose leaves survived a natural late frost in the field were screened for high maximum photosystem II efficiency (Fv/Fm) following chilling weather, as an indicator of their capacity for light-limited photosynthesis. Those showing the highest Fv/Fm were transferred to a high-light-controlled environment and maintained at chilling temperatures, where they were further screened for their capacities for high-light-limited and light-saturated leaf uptake of CO2CO2,max and Asat, respectively). Key Results For the first time, relatives of Mxg with significantly superior capacities for photosynthesis at chilling temperatures were identified. Msa accession '73/2' developed leaves in the spring that survived night-time frost, and during growth under chilling maintained a statistically significant 79 % higher ΦCO2,max, as a measure of light-limited photosynthesis, and a 70 % higher Asat, as a measure of light-saturated photosynthesis. A second Msa accession, '73/3' also showed significantly higher rates of leaf uptake of CO2. Conclusions As remarkable as Mxg has proved in its chilling tolerance of C4 photosynthesis, this study shows that there is still value and potential in searching for yet more superior tolerance. Msa accession '73/2' shows rates of light-limited and light-saturated photosynthesis at chilling temperatures that are comparable with those of the most cold-tolerant C3 species. This adds further proof to the thesis that C4 photosynthesis is not inherently limited to warm climates.

Original languageEnglish (US)
Pages (from-to)981-990
Number of pages10
JournalAnnals of Botany
Volume115
Issue number6
DOIs
StatePublished - May 1 2015

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C4 photosynthesis
Miscanthus
germplasm conservation
cold tolerance
screening
photosynthesis
frost
leaves
uptake mechanisms
temperature
C3 plants
photosystem II
cold treatment
weather
Japan
clones
grasses
climate
genotype

Keywords

  • Bioenergy crops
  • C photosynthesis
  • Miscanthus ;× giganteus
  • Miscanthus sacchariflorus
  • Miscanthus sinensis
  • Miscanthus tinctorius
  • chilling tolerance
  • light-limited photosynthesis
  • light-saturated photosynthesis.

ASJC Scopus subject areas

  • Plant Science

Cite this

Can the exceptional chilling tolerance of C4 photosynthesis found in Miscanthus ;× giganteus be exceeded? Screening of a novel Miscanthus Japanese germplasm collection. / Głowacka, Katarzyna; Jørgensen, Uffe; Kjeldsen, Jens B.; Kørup, Kirsten; Spitz, Idan; Sacks, Erik J.; Long, Stephen P.

In: Annals of Botany, Vol. 115, No. 6, 01.05.2015, p. 981-990.

Research output: Contribution to journalArticle

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abstract = "Background and Aims A clone of the hybrid perennial C4 grass Miscanthus ;× giganteus (Mxg) is known for achieving exceptionally high rates of leaf CO2 uptake during chilling. This is a requisite of success in the early spring, as is the ability of the leaves to survive occasional frosts. The aim of this study was to search for genotypes with greater potential than Mxg for photosynthesis and frost survival under these conditions. Methods A total of 864 accessions representing 164 local populations of M. sacchariflorus (Msa), M. sinensis (Msi) and M. tinctorius (Mti) collected across Japan were studied. Accessions whose leaves survived a natural late frost in the field were screened for high maximum photosystem II efficiency (Fv/Fm) following chilling weather, as an indicator of their capacity for light-limited photosynthesis. Those showing the highest Fv/Fm were transferred to a high-light-controlled environment and maintained at chilling temperatures, where they were further screened for their capacities for high-light-limited and light-saturated leaf uptake of CO2 (ΦCO2,max and Asat, respectively). Key Results For the first time, relatives of Mxg with significantly superior capacities for photosynthesis at chilling temperatures were identified. Msa accession '73/2' developed leaves in the spring that survived night-time frost, and during growth under chilling maintained a statistically significant 79 {\%} higher ΦCO2,max, as a measure of light-limited photosynthesis, and a 70 {\%} higher Asat, as a measure of light-saturated photosynthesis. A second Msa accession, '73/3' also showed significantly higher rates of leaf uptake of CO2. Conclusions As remarkable as Mxg has proved in its chilling tolerance of C4 photosynthesis, this study shows that there is still value and potential in searching for yet more superior tolerance. Msa accession '73/2' shows rates of light-limited and light-saturated photosynthesis at chilling temperatures that are comparable with those of the most cold-tolerant C3 species. This adds further proof to the thesis that C4 photosynthesis is not inherently limited to warm climates.",
keywords = "Bioenergy crops, C photosynthesis, Miscanthus ;× giganteus, Miscanthus sacchariflorus, Miscanthus sinensis, Miscanthus tinctorius, chilling tolerance, light-limited photosynthesis, light-saturated photosynthesis.",
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AU - Kjeldsen, Jens B.

AU - Kørup, Kirsten

AU - Spitz, Idan

AU - Sacks, Erik J.

AU - Long, Stephen P.

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