cAMP-activated Na+ current of molluscan neurons is resistant to kinase inhibitors and is gated by cAMP in the isolated patch

L. C. Sudlow, R. C. Huang, D. J. Green, Rhanor Gillette

Research output: Contribution to journalArticle

Abstract

The cAMP-dependent Na+ current (I(Na,cAMP)) modulates excitability in many molluscan neurons. Rapid activation of I(Na,cAMP) by cyclic nucleotide, its ion dependence, and its blockade by divalent cations resemble cyclic nucleotide-activated cation currents in vertebrate photoreceptors and olfactory receptors, where activation has been found to be independent of kinase activity. We tested the phosphorylation dependence of I(Na,cAMP) in neurons of the feeding and locomotory networks of the predatory marine snail Pleurobranchaea. Identified neurons of pedal and buccal ganglia were axotomized for recording the I(Na,cAMP) response to iontophoretic injection of cAMP under two-electrode voltage clamp. Intracellular injections of specific peptide inhibitor of protein kinase A had no blocking effects on activation of I(Na,cAMP) by iontophoretic injection of cAMP. Inward single- channel currents were activated in excised inside-out patches during exposure to cAMP in salines without added ATP. Sodium was the major current carrying ion. Two distinct types of I(Na,cAMP) channel activity were observed, where opening probability and open times differed, but conductance was similar, 36.7 pS. These observations suggest that I(Na,cAMP) activation occurs by direct binding of cAMP to a regulatory site at the channel, rather than by phosphorylation.

Original languageEnglish (US)
Pages (from-to)5188-5193
Number of pages6
JournalJournal of Neuroscience
Volume13
Issue number12
StatePublished - Dec 22 1993

Fingerprint

Phosphotransferases
Cyclic Nucleotides
Neurons
Injections
Pleurobranchaea
Odorant Receptors
Phosphorylation
Ions
Vertebrate Photoreceptor Cells
Cheek
Divalent Cations
Snails
Cyclic AMP-Dependent Protein Kinases
Ganglia
Cations
Foot
Electrodes
Adenosine Triphosphate
Sodium
Peptides

Keywords

  • cAMP
  • cAMP-gated ion channels
  • kinase inhibitors
  • Pleurobranchaea

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

cAMP-activated Na+ current of molluscan neurons is resistant to kinase inhibitors and is gated by cAMP in the isolated patch. / Sudlow, L. C.; Huang, R. C.; Green, D. J.; Gillette, Rhanor.

In: Journal of Neuroscience, Vol. 13, No. 12, 22.12.1993, p. 5188-5193.

Research output: Contribution to journalArticle

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