Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter

Daniel B. Szymanski, Birong Liao, Raymond E. Zielinski

Research output: Contribution to journalArticle

Abstract

Many stimuli increase cytoplasmic Ca2+ concentrations as an early signal transduction event and alter the patterns of nuclear gene transcription, but the mechanisms by which Ca2+ signals are transduced to the nucleus are not known. This article shows that at least four DNA binding proteins from cauliflower nuclear extracts are also calmodulin (CaM) binding proteins. CaM enhances the binding of these proteins to a C/G-box sequence element in the Arabidopsis Cam-3 promoter. Binding to the C/G-box is enhanced preferentially by the CaM isoform encoded by Cam-3. However, it is not clear whether the effect is mediated directly by CaM or indirectly through the activity of a CaM-regulated protein phosphatase. CaM also binds recombinant TGA3 and enhances its binding to the same Cam-3 promoter element. These results are consistent with the idea that a Ca2+-mediated signaling pathway eliciting some changes in gene expression may consist of CaM, or a structurally related Ca2+ binding protein, and transcription factors.

Original languageEnglish (US)
Pages (from-to)1069-1077
Number of pages9
JournalPlant Cell
Volume8
Issue number6
DOIs
StatePublished - Jun 1 1996

Fingerprint

Brassica
nuclear proteins
cauliflower
calmodulin
Calmodulin
Nuclear Proteins
Arabidopsis
Protein Isoforms
promoter regions
Calmodulin-Binding Proteins
binding proteins
calcium
Phosphoprotein Phosphatases
DNA-Binding Proteins
DNA-binding proteins
Signal Transduction
Carrier Proteins
Transcription Factors
Gene Expression
signal transduction

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter. / Szymanski, Daniel B.; Liao, Birong; Zielinski, Raymond E.

In: Plant Cell, Vol. 8, No. 6, 01.06.1996, p. 1069-1077.

Research output: Contribution to journalArticle

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