Calcium site mutations in cadherin: Impact on adhesion and evidence of cooperativity

A. Prakasam, Y. H. Chien, V. Maruthamuthu, D. E. Leckband

Research output: Contribution to journalArticle

Abstract

This work describes quantitative force and bead aggregation measurements of the adhesion and binding mechanisms of canine E-cadherin mutants W2A, D134A, D103A, D216A, D325A, and D436A. The W2A mutation affects the formation of the N-terminal strand dimer, and the remaining mutations target calcium binding sites at the interdomain junctions. Surface force measurements show that the full ectodomain of canine E-cadherin forms two bound states that span two intermembrane gap distances. The outer bond coincides with adhesion between the N-terminal extracellular domains (EC1) and the inner bond corresponds to adhesion via extracellular domain 3 (EC3). The W2A, D103A, D134A, and D216A mutations all eliminated adhesion between the N-terminal domains, and they attenuated or nearly eliminated the inner bond. The W2A mutant, which does not destabilize the protein structure, attenuates binding via EC3, which is separated from the mutation by several hundred amino acids. This long-range effect suggests that the presence or absence of tryptophan-2 docking allosterically alters the adhesive function of distal sites on the protein. This finding appears to reconcile the multidomain binding mechanism with mutagenesis studies, which suggested that W2 is the sole binding interface. The effects of the calcium site mutations indicate that structural perturbations cooperatively impact large regions of the protein structure. However, the influence of the calcium sites on cadherin structure and function depends on their location in the protein.

Original languageEnglish (US)
Pages (from-to)6930-6939
Number of pages10
JournalBiochemistry
Volume45
Issue number22
DOIs
StatePublished - Jun 6 2006

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Cadherins
Adhesion
Calcium
Mutation
Proteins
Canidae
Mutagenesis
Force measurement
Surface measurement
Tryptophan
Dimers
Adhesives
Agglomeration
Binding Sites
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Calcium site mutations in cadherin : Impact on adhesion and evidence of cooperativity. / Prakasam, A.; Chien, Y. H.; Maruthamuthu, V.; Leckband, D. E.

In: Biochemistry, Vol. 45, No. 22, 06.06.2006, p. 6930-6939.

Research output: Contribution to journalArticle

Prakasam, A. ; Chien, Y. H. ; Maruthamuthu, V. ; Leckband, D. E. / Calcium site mutations in cadherin : Impact on adhesion and evidence of cooperativity. In: Biochemistry. 2006 ; Vol. 45, No. 22. pp. 6930-6939.
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