Cadmium affects the expression of metallothionein (MT) and glutathione peroxidase (GPX) mRNA in goldfish, Carassius auratus

Cheol Young Choi, Kwang Wook An, Erik Nelson, Hamid R. Habibi

Research output: Contribution to journalArticle

Abstract

Cadmium (Cd) is a widespread non-essential heavy metal that enters the aquatic environment as a result of natural and human-caused activities, including industrial effluent, mining, and agricultural runoff. In the present study, we investigated time and dose-related effect of CdCl2 on metallothionein (MT) and glutathione peroxidase (GPX) mRNA levels in a number of goldfish tissues, in vivo. Basal MT and GPX mRNA levels remained unchanged in the tissues tested throughout the experiment. Injection with CdCl2 significantly increased MT mRNA levels in the brain, liver, kidney and intestine in a dose-dependant manner at all time tested (6, 12, 24 and 36 h). We isolated the full length GPX cDNA from goldfish kidneys, and found it to contain 785 nucleotides, including an open reading frame, predicted to encode a protein of 142 amino acids. In contrast, injection with CdCl2 significantly decreased GPX mRNA levels in the liver and kidney in a time-, and dose-, dependant, and became undetectable after 12, 24 and 36 h. The findings provide molecular characterization of MT and GPX in goldfish and suggest that exposure to Cd results in significant physiological changes in goldfish.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume145
Issue number4
DOIs
StatePublished - May 1 2007
Externally publishedYes

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Goldfish
Metallothionein
Glutathione Peroxidase
Cadmium
Cadmium Chloride
Messenger RNA
Kidney
Liver
Agricultural runoff
Tissue
Injections
Heavy Metals
Human Activities
Open Reading Frames
Intestines
Effluents
Brain
Nucleotides
Complementary DNA
Amino Acids

Keywords

  • Cadmium
  • Carassius auratus
  • Gene expression
  • Glutathione peroxidase
  • Metallothionein

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Cadmium affects the expression of metallothionein (MT) and glutathione peroxidase (GPX) mRNA in goldfish, Carassius auratus. / Choi, Cheol Young; An, Kwang Wook; Nelson, Erik; Habibi, Hamid R.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 145, No. 4, 01.05.2007, p. 595-600.

Research output: Contribution to journalArticle

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