Abstract

These studies quantified the relative effects of E-cadherin expression and homophilic ligation on the integrinmediated motility of epithelial cells. Micropatterned proteins were used to quantitatively titrate the ligation of E-cadherin and integrin receptors in order to assess their coordinate influence on the migration velocities of MDAMB-231 breast tumor epithelial cells. Fibronectin, E-cadherin, and mixtures of fibronectin and E-cadherin were covalently patterned on solid surfaces at defined compositions and mass coverages. The migration velocities of parental epithelial cells and of cells engineered to express E-cadherin under tetracycline control show that E-cadherin expression reduces cell motility by both adhesion-dependent and adhesion-independent mechanisms. Increasing E-cadherin expression levels also suppresses the dependence of cell velocity on the fibronectin coverage. On E-cadherin-containing substrata, the cell velocity decreases both with the E-cadherin expression level and with the immobilized E-cadherin surface density. These studies thus identified conditions under which E-cadherin preferentially suppresses cell migration by adhesion-independent versus adhesion-dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)10092-10099
Number of pages8
JournalLangmuir
Volume25
Issue number17
DOIs
StatePublished - Sep 1 2009

Fingerprint

Cadherins
Integrins
Adhesion
adhesion
cells
locomotion
tetracyclines
Fibronectins
Tumors
solid surfaces
breast
Proteins
tumors
Epithelial Cells
proteins
Chemical analysis
Tetracycline

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Cadherin and integrin regulation of epithelial cell migration. / Silvestre, Jonathan; Kenis, Paul J.A.; Leckband, Deborah E.

In: Langmuir, Vol. 25, No. 17, 01.09.2009, p. 10092-10099.

Research output: Contribution to journalArticle

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