Ca lectin gene insertion has the structural features of a transposable element

Lila O. Vodkin, Patsy R. Rhodes, Robert B. Goldberg

Research output: Contribution to journalArticle

Abstract

The single gene Le1, coding for soybean seed lectin, was compared to le1, a naturally occurring mutant allele containing a 3.4 kb insertion within its coding region. Le1 is devoid of introns and produces a 1.0 kb mRNA. It codes for a signal sequence of 32 amino acids and a mature protein of 253 amino acids. With the exception of six single-base substitutions, the coding and flanking sequences in le1 are identical with those in the uninterrupted gene. The insertion termini are imperfect inverted repeats flanked by a 3 bp duplication of lectin target DNA. Inverted repeats within the lectin gene are located symmetrically with respect to the insertion site and are homologous to a region of the insertion termini. These molecular traits conform with the structural aspects of transposable elements in other organisms and imply some degree of site specificity.

Original languageEnglish (US)
Pages (from-to)1023-1031
Number of pages9
JournalCell
Volume34
Issue number3
DOIs
StatePublished - Oct 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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